The Mir-X Inducible miRNA System (Red) is a set of expression vectors that allows you to establish a Tet-regulated microRNA (miRNA) expression system in mammalian cells. An additional feature of the system is that a bright red fluorescent protein marker, mCherry, is coexpressed along with your miRNA, enabling you to verify miRNA expression in intact cells. Inducible expression of the mCherry/pre-miRNA transcript is accomplished by creating a double-stable cell line that expresses the Tet-On Advanced transactivator and that bears your customized pmRi-mCherry/miRNA vector, and then culturing the cell line in the presence of the tetracycline derivative, doxycycline.
Overview
- Track your inducible miRNA expression with fluorescent protein markers
- High fold-induction and low basal expression
Applications
- Tetracycline-inducible miRNA expression in mammalian cells
