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MflI
MflI (XhoII) restriction site:
(A/G) | GATC (T/C)
(T/C) CTAG | (A/G)
Supplied buffer: L
Effect of DNA methylation: enzyme activity is affected by dam methylase
Reaction temperature: 37°C
- Source: Microbacterium flavum
- Concentration: 4–12 units/µl
- Substrate for unit definition: N6-methyladenine free lambda DNA
- Each lot undergoes stringent quality testing including: overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
MflI (XhoII) restriction site:
(A/G) | GATC (T/C)
(T/C) CTAG | (A/G)
Supplied buffer: L
Effect of DNA methylation: enzyme activity is affected by dam methylase
Reaction temperature: 37°C
- Source: Microbacterium flavum
- Concentration: 4–12 units/µl
- Substrate for unit definition: N6-methyladenine free lambda DNA
- Each lot undergoes stringent quality testing including: overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
