Methylation-dependent Restriction Enzyme McrBC
Methylation-dependent Restriction Enzyme McrBC
Brand: Takara Bio.
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Methylation-dependent Restriction Enzyme McrBC
McrBC specifically cleaves DNA containing methyl cytosine (mC: 5-hydroxymethylcytosine, 5-methylcytosine, 4-methylcytosine) preceded by a purine nucleotide (Pu: A or G). DNA cleavage by McrBC requires at least two PumC sites within a distance of 40–2,000 bp.
Overview
- Source: recombinant proteins expressed in E. coli
- Concentration: 1 unit /µl
- Supplied buffer: McrBC
- Reaction temperature: 37°C
- Substrate for unit definition: pBR322
- Effect of DNA methylation: This enzyme specifically cleaves DNA containing methyl cytosine (mC : 5-hydroxymethylcytosine, 5-methylcytosine, 4-methylcytosine) preceded by a purine nucleotide (Pu: A or G). DNA cleavage by McrBC requires at least two PumC sites within a distance of 40–2,000 bp.
Applications
- DNA methylation analysis (qAMP: quantitative analysis of DNA methylation using real-time PCR)
- Enrichment of unmethylated DNA (CHARM: Comprehensive high-throughput arrays for relative methylation)
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