MazF Single-protein Production
MazF Single-protein Production
Brand: Takara Bio.
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MazF Single-protein Production
The SPP (Single Protein Production) System utilizes a modified version of Takara's cold shock expression vectors along with expression of the endoribonuclease mazF to achieve remarkable protein purity and stable isotopic labeling of target proteins. Using this system, up to 90% of newly expressed protein is labelled recombinant target protein.
Principle of the SPP System: The mazF protein is a sequence-specific endoribonuclease that specifically cleaves single stranded RNAs at 5'-ACA-3' sequences. Expression of mazF virtually eliminates expression of most cellular mRNAs, while allowing synthesis of a target protein for up to 72 hours after induction. The SPP System consists of two co-expressed plasmids: 1) an ACA-less pCold expression vector, for expression of an ACA-less version of the target gene; and 2) a vector carrying the mazF gene, for inducible expression of the mazF protein. This system suppresses host protein expression more effectively than the pCold system alone, and results in higher purity and labeling efficiency for a wider variety of recombinant proteins. This system is markedly well suited for protein structural analysis by NMR (nuclear magnetic resonance) or other technologies.
Note: Licensing agreement is required. Please see our website for information. This product is for research purposes only. It must not be used for clinical purpose or in vitro diagnosis.
Overview
Principle of the SPP system
The MazF (mRNA Interferase) protein is a sequence-specific endoribonuclease that specifically cleaves single-stranded RNAs at their ACA sequences. As a result, the expression of MazF virtually eliminates expression of most cellular mRNAs while allowing synthesis of the target protein for up to 72 hr post-induction. The SPP system for cold-shock expression consists of two co-expressed plasmids: an ACA-less pCold expression vector for expression of an ACA-less version of the target gene, and a vector carrying the MazF gene for inducible expression of the MazF protein. This system suppresses host protein expression more effectively than the pCold system alone, resulting in higher purity and labeling efficiency for a wider variety of desired proteins. This also enables soluble protein expression applications such as structural analysis by NMR (nuclear magnetic resonance) or other technologies.
The SPP system was developed by Takara Bio in collaboration with Dr. Masayori Inouye at the University of Medicine and Dentistry of New Jersey, USA.
Applications
- Purified protein expression
- Radioisotope labeling (up to 90% of newly expressed cellular protein is labeled target protein)
- NMR analysis
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