Marathon cDNA Amplification Kit
Marathon cDNA Amplification Kit
Brand: Takara Bio.
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Marathon cDNA Amplification Kit
This kit contains reagents for making adaptor-ligated ds cDNA from poly A+ RNA and performing 5' and 3' Marathon RACE PCR using gene-specific primers and a mixture of thermostable DNA polymerases supplied by the user. This kit contains sufficient reagents for 5 cDNA synthesis reactions and 100 PCR reactions.
Overview
Marathon cDNA Amplification Kit (Cat. # 634913)
- Quickly obtain cDNAs without library screening
- Avoid troublesome single-stranded ligation or tailing reactions
- Perform 5' and 3' RACE from the same template
Applications
- RACE (Rapid Amplification of cDNA Ends)
- Identification of 3'/5' UTRs
- Full-length gene cloning
- Gene cloning and analysis
Marathon cDNA amplification of abundant (actin, 1.9 kb) and moderately rare (TFR, 5.1 kb) transcripts. The 5'- and 3'-RACE reactions for actin and TFR were performed with adaptor-ligated ds cDNA made from 1 μg of human placental poly A+ RNA and amplified for 25 PCR cycles. Full-length cDNAs were end-to-end PCR–amplified according to the User Manual. Lane 1: 1.2-kb actin 5'-RACE product. Lane 2: 1.3-kb actin 3'-RACE product. Lane 3: full-length 1.9-kb actin cDNA. Lane 4: 2.6- kb TFR 5'-RACE product. Lane 5: 2.9-kb TFR 3'-RACE product. Lane 6: full-length 5.1-kb TFR cDNA. Lane M: 1-kb DNA size ladder.
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Marathon cDNA amplification of abundant (actin, 1.9 kb) and moderately rare (TFR, 5.1 kb) transcripts. The 5'- and 3'-RACE reactions for actin and TFR were performed with adaptor-ligated ds cDNA made from 1 μg of human placental poly A+ RNA and amplified for 25 PCR cycles. Full-length cDNAs were end-to-end PCR–amplified according to the User Manual. Lane 1: 1.2-kb actin 5'-RACE product. Lane 2: 1.3-kb actin 3'-RACE product. Lane 3: full-length 1.9-kb actin cDNA. Lane 4: 2.6- kb TFR 5'-RACE product. Lane 5: 2.9-kb TFR 3'-RACE product. Lane 6: full-length 5.1-kb TFR cDNA. Lane M: 1-kb DNA size ladder.
