M13mp18 RF Phage Vector
M13mp18 RF Phage Vector
Brand: Takara Bio.
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M13mp18 RF Phage Vector
M13mp18 RF phage vector
The M13mp18 RF phage vector is suitable for M13 sequencing by the dideoxy sequencing method. Since the phage vector contains a multiple cloning site in the lacZ region, recombinant vectors can be easily verified via blue/white colony screening using agar plates containing IPTG and X-Gal. This phage vector can also directly be used in M13 sequencing reactions because it is processed as a single-stranded DNA from phage particles.
Purity
- >70% double-stranded covalently closed circular form I (RF I) DNA
- Multiple cloning site integrity verified by dideoxy sequencing analysis
- EcoRI, SacI, KpnI, SmaI, BamHI, XbaI, SalI, PstI, SphI, and HindIII restriction sites verified by restriction enzyme analysis
Storage
–20°C
Form
10 mM Tris-HCl (pH 8.0), 1 mM EDTA
GenBank
- Entry name: M13MP18, Accession No.: X02513
Concentration
0.5 µg/µl
M13 mp18 RF DNA Vector Map
M13 mp18 RF DNA Vector Map.
Product citations
Messing, J. New M13 vectors for cloning. Methods in Enzymology 101, 20–78 (1983).
Yanisch-Perron, C. et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33, 103–119 (1985).
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Purity
- >70% double-stranded covalently closed circular form I (RF I) DNA
- Multiple cloning site integrity verified by dideoxy sequencing analysis
- EcoRI, SacI, KpnI, SmaI, BamHI, XbaI, SalI, PstI, SphI, and HindIII restriction sites verified by restriction enzyme analysis
Storage
–20°C
Form
10 mM Tris-HCl (pH 8.0), 1 mM EDTA
GenBank
- Entry name: M13MP18, Accession No.: X02513
Concentration
0.5 µg/µl
M13 mp18 RF DNA Vector Map
M13 mp18 RF DNA Vector Map.
