M-SAN High Quality for Bioprocessing is a nonspecific endonuclease that is highly active at physiological conditions. M-SAN High Quality can be used directly in expression media, which can improve efficiency and yield in various workflows.
The Main Advantages of M-SAN High Quality
High purity (≥ 98%)
No protease detected
Animal origin-free production
Endotoxin-tested
High activity at physiological salt and pH
Supplied with extended product documentation
Properties
Source: Recombinantly produced in Pichia pastoris
Size: Protein size is 24.5 kDa.
pI: 8.62.
Unit definition: One Unit is defined as an increase in absorbance at 260 nm of 1 A in 30 minutes at 37°C, using 50 μg/ml calf thymus DNA in a buffer consisting of 25 mM Tris-HCl pH 7.6 @ 25°C, 2.5 mM MgCl2, 150 mM NaCl.
Activity:
Tmp: 25–40°C, optimal 37°C
NaCl: Working range (>20%): 50-400mM. Optimal range (>80%): 125mM–250mM
Mg2+ (>1mM) is required for activity, optimal range Mg2+ is >4 mM
pH: Working range (>20%): 6.5-10.0. Optimal range (>80%): 7.2–9.5
Tolerance to typical buffer additives:
DTT and other reducing agents may inactivate M-SAN HQ
Figure 1 M-SAN HQ has high activity in the 125 – 250 mM salt range. Activity was measured at 37°C in 25 mM Tris-HCl pH 7.4 @ 37°C, 2.5 mM MgCl2, 50 µg/ml calf thymus DNA.
Figure 2 M-SAN HQ has high activity over a broad pH range. Activity (ΔAbs260) was measured at 37°C in 25 mM Tris-HCl (blue) or 25 mM Bis-Tris (gray), 150 mM NaCl, 2.5 mM MgCl2, 50 µg/ml calf thymus DNA.
M-SAN High Quality for Bioprocessing is a nonspecific endonuclease that is highly active at physiological conditions. M-SAN High Quality can be used directly in expression media, which can improve efficiency and yield in various workflows.
The Main Advantages of M-SAN High Quality
High purity (≥ 98%)
No protease detected
Animal origin-free production
Endotoxin-tested
High activity at physiological salt and pH
Supplied with extended product documentation
Properties
Source: Recombinantly produced in Pichia pastoris
Size: Protein size is 24.5 kDa.
pI: 8.62.
Unit definition: One Unit is defined as an increase in absorbance at 260 nm of 1 A in 30 minutes at 37°C, using 50 μg/ml calf thymus DNA in a buffer consisting of 25 mM Tris-HCl pH 7.6 @ 25°C, 2.5 mM MgCl2, 150 mM NaCl.
Activity:
Tmp: 25–40°C, optimal 37°C
NaCl: Working range (>20%): 50-400mM. Optimal range (>80%): 125mM–250mM
Mg2+ (>1mM) is required for activity, optimal range Mg2+ is >4 mM
pH: Working range (>20%): 6.5-10.0. Optimal range (>80%): 7.2–9.5
Tolerance to typical buffer additives:
DTT and other reducing agents may inactivate M-SAN HQ
Figure 1 M-SAN HQ has high activity in the 125 – 250 mM salt range. Activity was measured at 37°C in 25 mM Tris-HCl pH 7.4 @ 37°C, 2.5 mM MgCl2, 50 µg/ml calf thymus DNA.
Figure 2 M-SAN HQ has high activity over a broad pH range. Activity (ΔAbs260) was measured at 37°C in 25 mM Tris-HCl (blue) or 25 mM Bis-Tris (gray), 150 mM NaCl, 2.5 mM MgCl2, 50 µg/ml calf thymus DNA.