Living Colors mCherry Monoclonal Antibody
Living Colors mCherry Monoclonal Antibody
Brand: Takara Bio.
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Living Colors mCherry Monoclonal Antibody
The Living Colors mCherry Monoclonal Antibody is Protein A-purified and was raised against mCherry. It recognizes mCherry, mOrange, mOrange2, mPlum, and mStrawberry, as well as C- and N-terminal fusions containing these fluorescent proteins.
Overview
- Monoclonal and polyclonal antibodies to detect common red fluorescent proteins, including mCherry, DsRed variants, AsRed, and tdTomato
- The mCherry Monoclonal Antibody can be used to distinguish mCherry from DsRed-Express or tdTomato
- Used for immunoprecipitation and immunolabeling applications in numerous studies
- Click the link to visit our red fluorescent protein vectors page
NOTE: The mCherry Monoclonal Antibody can be used to distinguish mCherry from DsRed-Express or tdTomato, neither of which are detected by the mCherry antibody.
Living Colors Fluorescent Protein antibody sources and dilutions for western blot, immunoprecipitation (IP) and immunocytochemistry (IC)
Living Colors Fluorescent Protein antibody sources and dilutions for western blot, immunoprecipitation (IP) and immunocytochemistry (IC).
The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells
The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells. HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates (corresponding to 30,000 cells) were prepared from HEK 293 cells transiently expressing PAmCherry-N1 (Lane 1) or a negative control (untransfected cells; Lane 2). Both lysates and a positive control (5 ng recombinant mCherry; Lane 3) were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000.
The mCherry Monoclonal Antibody differentiates red fluorescent proteins
The mCherry Monoclonal Antibody differentiates red fluorescent proteins. HEK293 cells were transiently transfected with vectors encoding the following fluorescent proteins, and then analyzed by Western blot with the mCherry Monoclonal Antibody (0.5 mg/ml; 1:1000 dilution). Panel A. The mCherry Monoclonal Antibody recognizes mCherry but not tdTomato, DsRed-Express2, or DsRed-Monomer. Lane 1: control (untransfected cells), Lane 2: mCherry, Lane 3: tdTomato, Lane 4: DsRed-Monomer, Lane 5: DsRed-Express2. Panel B. The mCherry Monoclonal Antibody differentiates some Fruit Fluorescent Proteins. It detects mCherry, mOrange, mPlum, and mStrawberry, but not mRaspberry or tdTomato. mBanana is only faintly detected. Lane 1: control (untransfected cells), Lane 2: mCherry, Lane 3: mBanana, Lane 4: mOrange, Lane 5: mPlum, Lane 6: mRaspberry, Lane 7: mStrawberry, Lane 8: tdTomato. Exposure times, 15 sec and 5 min, respectively. Lanes M, marker.
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Living Colors Fluorescent Protein antibody sources and dilutions for western blot, immunoprecipitation (IP) and immunocytochemistry (IC)
Living Colors Fluorescent Protein antibody sources and dilutions for western blot, immunoprecipitation (IP) and immunocytochemistry (IC).
The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells
The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells. HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates (corresponding to 30,000 cells) were prepared from HEK 293 cells transiently expressing PAmCherry-N1 (Lane 1) or a negative control (untransfected cells; Lane 2). Both lysates and a positive control (5 ng recombinant mCherry; Lane 3) were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000.
The mCherry Monoclonal Antibody differentiates red fluorescent proteins
The mCherry Monoclonal Antibody differentiates red fluorescent proteins. HEK293 cells were transiently transfected with vectors encoding the following fluorescent proteins, and then analyzed by Western blot with the mCherry Monoclonal Antibody (0.5 mg/ml; 1:1000 dilution). Panel A. The mCherry Monoclonal Antibody recognizes mCherry but not tdTomato, DsRed-Express2, or DsRed-Monomer. Lane 1: control (untransfected cells), Lane 2: mCherry, Lane 3: tdTomato, Lane 4: DsRed-Monomer, Lane 5: DsRed-Express2. Panel B. The mCherry Monoclonal Antibody differentiates some Fruit Fluorescent Proteins. It detects mCherry, mOrange, mPlum, and mStrawberry, but not mRaspberry or tdTomato. mBanana is only faintly detected. Lane 1: control (untransfected cells), Lane 2: mCherry, Lane 3: mBanana, Lane 4: mOrange, Lane 5: mPlum, Lane 6: mRaspberry, Lane 7: mStrawberry, Lane 8: tdTomato. Exposure times, 15 sec and 5 min, respectively. Lanes M, marker.
