Linear Hygromycin Marker
Linear Hygromycin Marker
Brand: Takara Bio.
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Linear Hygromycin Marker
Linear Hygromycin Marker is a short ds DNA fragment consisting of the hygromycin resistance gene regulated by the SV40 promoter and ending with the SV40 polyadenylation signal. The 2.1-kb marker is compatible with any expression vector (including Tet System vectors) for generating stable transfectants using CalPhos transfection reagent or a liposome-mediated transfection protocol.
Overview
- Both the linear selection markers and the pTK-Hyg Vector and pPur Vector may be used in cotransfections to establish stable cell lines
- Linear selection markers require you to screen fewer clones, resulting in more positive clones than when circular plasmid markers are used
- Linear selection markers are ideal for use with all Takara Bio's Tet-On and Tet-Off Inducible Expression Systems—screen fewer candidates and find more highly inducible clones
Cotransfection of a hygromycin or puromycin linear selection marker achieves a higher number of positive clones than using either a single response vector containing a selection marker, or cotransfecting with a circular selection marker
Cotransfection of a hygromycin or puromycin linear selection marker achieves a higher number of positive clones than using either a single response vector containing a selection marker, or cotransfecting with a circular selection marker.
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Cotransfection of a hygromycin or puromycin linear selection marker achieves a higher number of positive clones than using either a single response vector containing a selection marker, or cotransfecting with a circular selection marker
Cotransfection of a hygromycin or puromycin linear selection marker achieves a higher number of positive clones than using either a single response vector containing a selection marker, or cotransfecting with a circular selection marker.
