Lentiviral Vectors with Fluorescent Proteins
Lentiviral Vectors with Fluorescent Proteins
Brand: Takara Bio.
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Lentiviral Vectors with Fluorescent Proteins
These lentiviral vectors allow you to deliver and stably express your gene of interest as a fluorescent fusion protein in virtually any cell type, including dividing and nondividing cells, stem cells, terminally differentiated cells, and neuronal cells. Three bicistronic lentiviral vectors (pLVX-IRES-mCherry, pLVX-IRES-tdTomato, and pLVX-IRES-ZsGreen1) are also available to allow your gene of interest and a bright fluorescent protein to be coexpressed from a single mRNA transcript. Takara Bio’s Living Colors DsRed-Monomer, AcGFP1, and mCherry proteins are truly monomeric fluorescent proteins that are ideal for use as fusion tags. ZsGreen1 and tdTomato are supremely bright fluorescent proteins that are excellent reporters for gene expression, or for measuring transduction/transfection efficiency. mCherry is a very popular fluorescent protein derived by directed mutagenesis of mRFP1, a monomeric mutant of DsRed. DsRed-Express2 is a rapidly maturing variant of Discosoma sp. red fluorescent protein (DsRed). It is highly soluble and was designed to be better suited for cell and stem cell applications.
All Living Colors proteins are detectable without added cofactors or substrates, making them exceptional, noninvasive tools for live-cell studies.
DsRed-Monomer & AcGFP1
- Truly monomeric proteins (see DsRed-Monomer for more information)
- Ideal for tagging proteins for trafficking and localization studies
- Distinct spectra make them excellent choices for double labeling experiments
ZsGreen1 & tdTomato
- The brightest green and red fluorescent proteins—(2.5X and 3.5X brighter than EGFP, respectively)
- IRES bicistronic expression for monitoring transduction efficiency
- Separate expression of your cDNA and a fluorescent protein from a single transcript
mCherry
- A bright monomeric protein
- Matures very rapidly—its t1/2 for maturation at 37°C is approximately 15 minutes
DsRed-Express2
- Rapidly maturing red fluorescent protein with enhanced solubility and reduced green emission
- Well-suited for cell and stem cell applications
Overview
- Monitor the expression of your lentiviral-delivered genes with fluorescent tags and markers.
- Visualize your protein using fluorescence microscopy
- Generate both N- & C-terminal fusions to bright fluorescent proteins
- Learn more from our fluorescent protein quick guide
- For the highest titers use in combination with Lenti-X Packaging Single Shots (VSV-G) and Lenti-X 293T Cell Line
Lentiviral vectors with fluorescent proteins
Lentiviral vectors with fluorescent proteins. Lenti-X vectors contain sequence elements that facilitate lentiviral packaging and/or boost transgene expression, including the LTRs, packaging signal (Ψ), Rev response element (RRE), and central polypurine tract/central termination sequence (cPPT/CTS) from HIV-1; and the woodchuck hepatitis virus post-transcriptional regulatory element (WPRE). Vectors can express your protein fused at its N- or C- terminus to either a green (AcGFP1) or red (DsRed-Monomer) fluorescent protein tag, or coexpress it as a separate protein along with ZsGreen1 (shown), mCherry, or tdTomato.
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Lentiviral vectors with fluorescent proteins
Lentiviral vectors with fluorescent proteins. Lenti-X vectors contain sequence elements that facilitate lentiviral packaging and/or boost transgene expression, including the LTRs, packaging signal (Ψ), Rev response element (RRE), and central polypurine tract/central termination sequence (cPPT/CTS) from HIV-1; and the woodchuck hepatitis virus post-transcriptional regulatory element (WPRE). Vectors can express your protein fused at its N- or C- terminus to either a green (AcGFP1) or red (DsRed-Monomer) fluorescent protein tag, or coexpress it as a separate protein along with ZsGreen1 (shown), mCherry, or tdTomato.
