Lentiviral Vector Systems with an EF-1 alpha promoter
Lentiviral Vector Systems with an EF-1 alpha promoter
Brand: Takara Bio.
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Lentiviral Vector Systems with an EF-1 alpha promoter
Lenti-X lentiviral vector systems with EF-1 alpha promoters allow you to achieve robust, constitutive, long-term expression of your gene of interest in cell types in which CMV promoters are often silenced, such as hematopoietic and stem cells. The EF-1 alpha promoter, which offers a broad host range, is derived from the human EEF1A1 gene that expresses the alpha subunit of eukaryotic elongation factor 1 (Uetsuki et al. 1989; Wan Kim et al. 1990).
Options for lentiviral vectors with an EF-1 alpha promoter
The Lenti-X Expression System (EF1α Version) is a complete system that includes the pLVX-EF1α-IRES-Puro Vector, and our fourth-generation lentiviral packaging system. Alternatively, you can express your protein fused to a red (mCherry or DsRed-Monomer) or green (AcGFP1) fluorescent protein, or express your protein and a fluorescent protein separately from an internal ribosome entry site (IRES).
Overview
- High-titer lentiviral vector system
- Constitutive expression from the human EF1 alpha promoter
- Robust, long-term expression, in the broadest range of cell types
- Bright fluorescent proteins to monitor expression or localization, learn more from our fluorescent protein quick guide.
Applications
- If CMV promoters are silenced in your cell type, try EF-1 alpha
- Lentiviral transduction of stem cells
- Lentiviral transduction of hematopoietic cells
4th generation lentiviral packaging system
Fourth-generation lentiviral packaging system. A lentiviral vector and the Lenti-X HTX Packaging Mix are cotransfected into 293T cells. High titer lentiviral supernatants are ready for use 48 hr after transfection.
Lentiviral vector systems with EF-1 alpha promoters
Lentiviral vector systems with EF-1 alpha promoters. Achieve robust, constitutive, long-term expression of your gene of interest in cell types in which CMV promoters are often silenced, such as hematopoietic and stem cells.
Expression of AcGFP1 driven by the EF-1 alpha promoter in stem cell lines is higher than expression driven by the CMV promoter
Expression of AcGFP1 driven by the EF-1 alpha promoter in stem cell lines is higher than expression driven by the CMV promoter. The mouse embryonic stem cell lines E14 (Panel A) and D3 (Panel B) were transduced by Lenti-X lentivirus, expressing AcGFP1 either under the control of the CMV promoter or the Elongation factor alpha (EF-1 alpha) promoter. The expression level of AcGFP1 in infected cells five days postinfection was monitored by FACS analysis using the FL1 channel. The expression of AcGFP1 driven by the EF-1 alpha promoter in both stem cell lines was considerably higher compared to the CMV promoter. This is mainly due to a considerably lower rate of silencing of the EF-1 alpha promoter in stem cells compared to the CMV promoter as published (Wang, et al. (2008) Stem Cells Dev 17:279–289).
Uetsuki, T., Naito, A., Nagata, S. & Kaziro, Y. Isolation and characterization of the human chromosomal gene for polypeptide chain elongation factor-1. J. Biol. Chem. 264, 5791–5798 (1989).
Wan Kim, D., Uetsuki, T., Kaziro, Y., Yamaguchi, N. & Sugano, S. Use of the human alongation factor 1a promoter as a versatile and efficient expression system. Gene 91, 217–223 (1990).
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4th generation lentiviral packaging system
Fourth-generation lentiviral packaging system. A lentiviral vector and the Lenti-X HTX Packaging Mix are cotransfected into 293T cells. High titer lentiviral supernatants are ready for use 48 hr after transfection.
Lentiviral vector systems with EF-1 alpha promoters
Lentiviral vector systems with EF-1 alpha promoters. Achieve robust, constitutive, long-term expression of your gene of interest in cell types in which CMV promoters are often silenced, such as hematopoietic and stem cells.
Expression of AcGFP1 driven by the EF-1 alpha promoter in stem cell lines is higher than expression driven by the CMV promoter
Expression of AcGFP1 driven by the EF-1 alpha promoter in stem cell lines is higher than expression driven by the CMV promoter. The mouse embryonic stem cell lines E14 (Panel A) and D3 (Panel B) were transduced by Lenti-X lentivirus, expressing AcGFP1 either under the control of the CMV promoter or the Elongation factor alpha (EF-1 alpha) promoter. The expression level of AcGFP1 in infected cells five days postinfection was monitored by FACS analysis using the FL1 channel. The expression of AcGFP1 driven by the EF-1 alpha promoter in both stem cell lines was considerably higher compared to the CMV promoter. This is mainly due to a considerably lower rate of silencing of the EF-1 alpha promoter in stem cells compared to the CMV promoter as published (Wang, et al. (2008) Stem Cells Dev 17:279–289).
