Lentiviral Vector Systems for Constitutive Gene Expression
Lentiviral Vector Systems for Constitutive Gene Expression
Brand: Takara Bio.
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Lentiviral Vector Systems for Constitutive Gene Expression
These lentiviral expression systems are designed for constitutive gene expression from either a CMV or EF1-alpha promoter.
Biscistronic options
The IRES Vectors included with the bicistronic lentiviral expression systems each contain a multiple cloning site located upstream of the IRES from which a selection marker is expressed. Because your gene of interest and a selection marker are translated from a single RNA, you can be certain that nearly 100% of the colonies that are resistant to puromycin, hygromycin, and G418 also express your protein of interest.
Fluorescent protein options
For lentiviral vectors that express fluorescent protein tags and markers, including the bicistronic pLVX-IRES-ZsGreen1 and pLVX-IRES-mCherry vectors, see our Fluorescent Lentiviral Expression Vectors webpage.
EF1-alpha promoter versions
Lenti-X lentiviral vector systems with EF1-alpha promoters allow you to achieve robust, constitutive, long-term expression of your gene of interest in cell types in which CMV promoters are often silenced, such as hematopoietic and stem cells.
Lentiviral packaging system included
Many of our lentiviral systems are complete systems that include our high-titer Lenti-X Packaging Single Shots (VSV-G) when used with the Lenti-X 293T Cell Line (not included), generates titers that are far superior to those of other commercially available lentiviral packaging systems. Frequently, an entire dish of target cells can be effectively transduced using just 10–100 µl of unconcentrated viral supernatant.
Overview
- Complete systems include a high-titer lentiviral packaging system and an optimized transfer vector
- Vector design optimized for high expression and high titers
- cPPT element enhances transduction efficiency
- WPRE enhances expression of your transgene and increases viral titer
- EF1-alpha promoter versions for cell types where CMV promoter may be silenced
- IRES-containing bicistronic vectors allow the simultaneous expression of your gene and a drug selection marker from the same RNA transcript
Lentiviral vector design
Lentiviral vector design. Clontech's lentiviral vectors contain sequence elements that facilitate lentiviral packaging and boost transgene expression. The Lenti-X Expression System (pLVX-Puro Vector) is used for constitutive expression of your transgene. The Lenti-X Bicistronic Expression Systems allow your transgene and a selectable marker to be coexpressed from a single mRNA transcript.
High-titer lentivirus production
High-titer lentivirus production. Lenti-X 293T cells were transduced with the indicated volumes (µl) of lentiviral packaging supernatant generated with the Lenti-X Expression System and then selected with puromycin for 9 days to allow the formation of the resistant colonies, which were then stained with crystal violet.
4th generation lentiviral packaging system
Fourth-generation lentiviral packaging system. A lentiviral vector and the Lenti-X HTX Packaging Mix are cotransfected into 293T cells. High titer lentiviral supernatants are ready for use 48 hr after transfection.
Lentiviral vector systems with EF-1 alpha promoters
Lentiviral vector systems with EF-1 alpha promoters. Achieve robust, constitutive, long-term expression of your gene of interest in cell types in which CMV promoters are often silenced, such as hematopoietic and stem cells.
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Lentiviral vector design
Lentiviral vector design. Clontech's lentiviral vectors contain sequence elements that facilitate lentiviral packaging and boost transgene expression. The Lenti-X Expression System (pLVX-Puro Vector) is used for constitutive expression of your transgene. The Lenti-X Bicistronic Expression Systems allow your transgene and a selectable marker to be coexpressed from a single mRNA transcript.
High-titer lentivirus production
High-titer lentivirus production. Lenti-X 293T cells were transduced with the indicated volumes (µl) of lentiviral packaging supernatant generated with the Lenti-X Expression System and then selected with puromycin for 9 days to allow the formation of the resistant colonies, which were then stained with crystal violet.
4th generation lentiviral packaging system
Fourth-generation lentiviral packaging system. A lentiviral vector and the Lenti-X HTX Packaging Mix are cotransfected into 293T cells. High titer lentiviral supernatants are ready for use 48 hr after transfection.
Lentiviral vector systems with EF-1 alpha promoters
Lentiviral vector systems with EF-1 alpha promoters. Achieve robust, constitutive, long-term expression of your gene of interest in cell types in which CMV promoters are often silenced, such as hematopoietic and stem cells.
