Lenti-X Tet-On 3G Inducible Expression System
Lenti-X Tet-On 3G Inducible Expression System
Brand: Takara Bio.
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Lenti-X Tet-On 3G Inducible Expression System
The Lenti-X Tet-On 3G Inducible Expression System is a tetracycline-inducible lentiviral gene expression system that allows you to produce high titers of recombinant, VSV-G-pseudotyped lentiviruses for the purpose of establishing a tightly inducible expression system for your gene of interest in both dividing and nondividing cell types. The system provides enough reagents for 16 lentiviral packaging reactions. In the presence of doxycycline (Dox), the Tet-On 3G transactivator specifically binds and activates high-level transcription from the inducible promoter that controls expression of your gene.
Overview
- Low basal expression, high maximal expression, highly controllable
- Lenti-X Packaging Single Shots (VSV-G), included with each system, generate high titers with a very high safety profile
- TRE3G promoter provides very tight control of transcription
- Tet-One formats are also available
Applications
- Tetracycline-inducible expression in the widest range of hard-to-transfect cell types.
The Tet-On 3G systems allow inducible gene expression only in the presence of doxycycline (Dox)
The Tet-On 3G systems allow inducible gene expression only in the presence of doxycycline (Dox). When Dox binds, the transactivator undergoes a conformational change allowing it to bind tet operator (tetO) repeats within the TRE3G promoter.
Inducible expression of luciferase using transduced lentivirus
Inducible expression of luciferase using transduced lentivirus. LVX-Tet3G and LVX-TRE3G-Luc lentivirus were generated with the Lenti-X HTX Packaging System (supplied) and used to co-infect HepG2 cells at a 1:1 ratio. After 24 hr, the transduced cells were treated with the indicated dose of doxycycline. Expression of luciferase was determined a further 48 hr later using an anti-luciferase antibody on a Western blot.
Lentiviral Tet-On 3G systems
Lentiviral Tet-On 3G systems. There are four systems available that express the Tet-On 3G transactivator from a CMV promoter.
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The Tet-On 3G systems allow inducible gene expression only in the presence of doxycycline (Dox)
The Tet-On 3G systems allow inducible gene expression only in the presence of doxycycline (Dox). When Dox binds, the transactivator undergoes a conformational change allowing it to bind tet operator (tetO) repeats within the TRE3G promoter.
Inducible expression of luciferase using transduced lentivirus
Inducible expression of luciferase using transduced lentivirus. LVX-Tet3G and LVX-TRE3G-Luc lentivirus were generated with the Lenti-X HTX Packaging System (supplied) and used to co-infect HepG2 cells at a 1:1 ratio. After 24 hr, the transduced cells were treated with the indicated dose of doxycycline. Expression of luciferase was determined a further 48 hr later using an anti-luciferase antibody on a Western blot.
Lentiviral Tet-On 3G systems
Lentiviral Tet-On 3G systems. There are four systems available that express the Tet-On 3G transactivator from a CMV promoter.
