Lenti-X qRT-PCR Titration Kit

Lenti-X qRT-PCR Titration Kit

Brand: Takara Bio.
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Lenti-X qRT-PCR Titration Kit
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Lenti-X™ qRT-PCR Titration Kit
SKU: 631235
200 Rxns
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Lenti-X qRT-PCR Titration Kit
Lenti-X qRT-PCR Titration Kit

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This Lenti-X qRT-PCR Titration Kit and a real-time PCR instrument can be used to rapidly quantify the virion content of HIV-1-based lentiviral supernatants. The viral genome copy number in a supernatant sample is determined by first purifying the viral RNA, and then comparing the RT-PCR threshold cycle (Ct) of an appropriate dilution of purified viral RNA to sample curve Ct values that are derived from a calibrated, serially diluted control viral RNA template.

Overview

  • Confirm the success of your lentiviral packaging reaction
  • Perform consistent experiments
  • Ensure sufficient MOIs so that 100% of your cells are transduced
  • Control copy number and hence expression levels
  • 200 qRT-PCR reactions are included*
  • Purify 10 different virus preps

Lenti-X and Retro-X qRT-PCR titration kits offer the fastest titration methods

Lenti-X and Retro-X qRT-PCR titration kits offer the fastest titration methods

Lenti-X and Retro-X qRT-PCR kitration kits offer the fastest titration methods. Standard transduction-based titration methods require up to 10 days to complete.The Lenti-X and Retro-X qRT-PCR titration kits require only 4 hr, which enables virus harvest, titration, and infection of target cells to all be performed on the same day.

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This Lenti-X qRT-PCR Titration Kit and a real-time PCR instrument can be used to rapidly quantify the virion content of HIV-1-based lentiviral supernatants. The viral genome copy number in a supernatant sample is determined by first purifying the viral RNA, and then comparing the RT-PCR threshold cycle (Ct) of an appropriate dilution of purified viral RNA to sample curve Ct values that are derived from a calibrated, serially diluted control viral RNA template.

Overview

  • Confirm the success of your lentiviral packaging reaction
  • Perform consistent experiments
  • Ensure sufficient MOIs so that 100% of your cells are transduced
  • Control copy number and hence expression levels
  • 200 qRT-PCR reactions are included*
  • Purify 10 different virus preps

Lenti-X and Retro-X qRT-PCR titration kits offer the fastest titration methods

Lenti-X and Retro-X qRT-PCR titration kits offer the fastest titration methods

Lenti-X and Retro-X qRT-PCR kitration kits offer the fastest titration methods. Standard transduction-based titration methods require up to 10 days to complete.The Lenti-X and Retro-X qRT-PCR titration kits require only 4 hr, which enables virus harvest, titration, and infection of target cells to all be performed on the same day.

Write Your Own Review
You're reviewing:Lenti-X qRT-PCR Titration Kit
Your Rating