This Lenti-X qRT-PCR Titration Kit and a real-time PCR instrument can be used to rapidly quantify the virion content of HIV-1-based lentiviral supernatants. The viral genome copy number in a supernatant sample is determined by first purifying the viral RNA, and then comparing the RT-PCR threshold cycle (Ct) of an appropriate dilution of purified viral RNA to sample curve Ct values that are derived from a calibrated, serially diluted control viral RNA template.
Overview
Confirm the success of your lentiviral packaging reaction
Perform consistent experiments
Ensure sufficient MOIs so that 100% of your cells are transduced
Lenti-X and Retro-X qRT-PCR titration kits offer the fastest titration methods
Lenti-X and Retro-X qRT-PCR kitration kits offer the fastest titration methods. Standard transduction-based titration methods require up to 10 days to complete.The Lenti-X and Retro-X qRT-PCR titration kits require only 4 hr, which enables virus harvest, titration, and infection of target cells to all be performed on the same day.
This Lenti-X qRT-PCR Titration Kit and a real-time PCR instrument can be used to rapidly quantify the virion content of HIV-1-based lentiviral supernatants. The viral genome copy number in a supernatant sample is determined by first purifying the viral RNA, and then comparing the RT-PCR threshold cycle (Ct) of an appropriate dilution of purified viral RNA to sample curve Ct values that are derived from a calibrated, serially diluted control viral RNA template.
Overview
Confirm the success of your lentiviral packaging reaction
Perform consistent experiments
Ensure sufficient MOIs so that 100% of your cells are transduced
Lenti-X and Retro-X qRT-PCR titration kits offer the fastest titration methods
Lenti-X and Retro-X qRT-PCR kitration kits offer the fastest titration methods. Standard transduction-based titration methods require up to 10 days to complete.The Lenti-X and Retro-X qRT-PCR titration kits require only 4 hr, which enables virus harvest, titration, and infection of target cells to all be performed on the same day.