The Lenti-X p24 Rapid Titer Kit allows you to quickly determine the titer of any HIV-1-based lentiviral vector supernatant using a standard ELISA. The wells of the included microtiter plate (12 x 8-well strips) are coated with anti-HIV-1 p24 capture antibody, which binds the p24 (an HIV-1 core protein) in your test samples. Specifically-bound p24 is detected in a typical "sandwich" ELISA format using a biotinylated anti-p24 secondary antibody, a streptavidin-HRP conjugate, and a color-producing substrate. Color intensity is measured spectrophotometrically to indicate the level of p24 in the samples, which is then quantified against a p24 standard curve in order to titrate the viral content.

Overview