Lenti-X Integration Site Analysis Kit
Lenti-X Integration Site Analysis Kit
Brand: Takara Bio.
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Lenti-X Integration Site Analysis Kit
The Lenti-X Integration Site Analysis Kit contains all the reagents needed for PCR-based identification of lentiviral vector integration sites in transduced cell clones. The first step is the construction of pools of adaptor-ligated genomic DNA fragments, referred to as "libraries". These libraries can then be used for identifying genomic DNA sequences flanking the 5' end of the integrated provirus using long-distance PCR.
Overview
- Identify sites of lentiviral integration into the host genome
- Simple determination of lentivirus integration sites using GenomeWalker technology
- Compatible with most commercially available lentivirus vectors
- Complete system including all the necessary enzymes, primers, controls, PCR kit, genomic DNA purification kit, and gel purification kit
- Retroviral version also available for detecting integration sites for MMLV and MSCV retroviruses
- Identify sites of lentiviral integration into the host genome
Flow chart of the Lenti-X integration site analysis protocol
Flow chart of the Lenti-X integration site analysis protocol. The major steps include construction of three viral integration libraries, PCR amplification of genomic DNA from the libraries, and analysis of PCR products. The gel shows a typical result. Lane 1: HpaI library. Lane 2: DraI library. Lane 3: SspI library. Lane 4: No template control. Lane M: DNA size markers. AP: Adaptor primer. LSP: Lentivirus-specific primer.
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Flow chart of the Lenti-X integration site analysis protocol
Flow chart of the Lenti-X integration site analysis protocol. The major steps include construction of three viral integration libraries, PCR amplification of genomic DNA from the libraries, and analysis of PCR products. The gel shows a typical result. Lane 1: HpaI library. Lane 2: DraI library. Lane 3: SspI library. Lane 4: No template control. Lane M: DNA size markers. AP: Adaptor primer. LSP: Lentivirus-specific primer.
