Lenti-X iDimerize Inducible Homodimer System
Lenti-X iDimerize Inducible Homodimer System
Brand: Takara Bio.
In stock
SKU
Lenti-X iDimerize Inducible Homodimer System
The Lenti-X iDimerize Inducible Homodimer Vector Set 1 is a component of the Lenti-X iDimerize Inducible Homodimer System (Cat. No. 635072). This system enables lentiviral delivery of your gene of interest into a variety of mammalian cells, and the expression of your protein of interest, tagged with a domain allowing for inducible protein homodimerization, via a small molecule "dimerizer".
The Lenti-X iDimerize Inducible Homodimer System (with Tet-On 3G) is a tetracycline-inducible lentiviral gene expression system that lets you control the expression level and dimerization of a protein of interest in live cells. The system includes the pLVX-TRE3G-Hom1 Vector Set, which contains a mammalian expression vector (pLVX-TRE3G-Hom1) that allows doxycycline-dependent expression of your gene of interest, tagged with the DmrB domain, in Tet-On 3G transactivator-expressing cells. Homodimerization of the resulting DmrB-tagged protein is controlled by the addition of the B/B Homodimerizer.
The Lenti-X iDimerize Inducible Homodimer System (with Tet-On 3G) includes vectors and linear selection markers, an optimized packaging system, and the B/B Homodimerizer. The system provides enough reagents for 16 lentiviral packaging reactions.
Overview
- Previously available from ARIAD as the ARGENT Regulated Homodimerization Kit
- Rapid chemical induction of cell signaling pathways
- Activate any pathway or cellular event controlled by homo-oligomers (e.g., dimers, trimers, or tetramers)
- Established tool used by over 2,000 research groups, with over 400 scientific publications to date
- B/B Homodimerizer is identical to the AP20187 ligand
- B/B Washout Ligand reverses protein oligomers that were induced by the B/B Homodimerizer
- Plasmid and lentiviral (Lenti-X) vector formats are available
Applications
- Induce any event or pathway that is controlled or activated by protein-protein interactions
- Activate signaling pathways in real time
- Study transmembrane receptors
- Recruit signaling proteins to specific subcellular locations (e.g., the plasma membrane)
- Inducible homodimer technology has been used for functional studies of:
- Receptor and non-receptor tyrosine kinases
- Receptor and non-receptor serine/threonine kinases
- Non-kinase receptors
- Signaling proteases
- Adaptor proteins
- Cell adhesion and rolling
- DNA looping
- Activation of recombinase
- RNA splicing
- Protein splicing
- Glycosylation
Burnett, S. H. et al. Conditional macrophage ablation in transgenic mice expressing a Fas-based suicide gene. J. Leukoc. Biol. 75, 612–623 (2004).
Freeman, K. W. et al. Inducible prostate intraepithelial neoplasia with reversible hyperplasia in conditional FGFR1-expressing mice. Cancer Res. 63, 8256–63 (2003).
Write Your Own Review
