Lenti-X iDimerize Inducible Heterodimer System
Lenti-X iDimerize Inducible Heterodimer System
Brand: Takara Bio.
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Lenti-X iDimerize Inducible Heterodimer System
The Lenti-X iDimerize Inducible Heterodimer System lets you control the heterodimerization of two different proteins of interest in live cells by adding a small molecule, the membrane-permeant compound A/C Heterodimerizer, to the culture medium. The system includes the Lenti-X iDimerize Inducible Heterodimer Vector Set 1, which contains four lentiviral vectors encoding dimerization tags (DmrA or DmrC, respectively), as well as subcellular localization tags that can be easily fused to your protein of interest. In combination with a lentiviral packaging system, the optimized Lenti-X constructs allow for packaging and delivery of high-titer lentivirus to the widest range of cell types. Using this system, the activity and/or localization of the resulting chimeric protein can be controlled by the addition of A/C Heterodimerizer, which is required for heterodimerization and is also included.
Overview
- Achieve small-molecule control of cellular events that are usually activated by protein heterodimerization
- Control the subcellular localization of a target protein
- A/C Heterodimerizer is identical to the AP21967 ligand
- For inducible control of transcription factors, use the iDimerize Regulated Transcription System
- Plasmid and lentiviral (Lenti-X) vector formats are available
- Previously available from ARIAD Pharmaceuticals, Inc., as the ARGENT Regulated Heterodimerization Kit
Applications
- Create and control interactions between two different proteins
- Create conditional alleles of receptors and signaling molecules
- Activate cell surface receptors or other signaling proteins
- Recruit signaling proteins to specific subcellular locations (e.g., the plasma membrane or nucleus)
- Reconstitute enzyme activity by inducing the interaction of separate polypeptides
- Functional analysis of a signaling pathway in multiple cell types
- Inducible protein dimerization in animal models and cultured cell lines
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