Lenti-X Concentrator
Lenti-X Concentrator
Brand: Takara Bio.
In stock
SKU
Lenti-X Concentrator
The Lenti-X Concentrator is a complete reagent for the concentration of infectious lentiviral vector particles. This reagent provides a scalable alternative to ultracentrifugation for viral particle concentration. Vector supernatants can be concentrated 10–100 fold, depending upon the volumes used.
Overview
- Simple mix-and-spin protocol
- Hassle-free and easily scaled up for large volumes
- No ultracentrifugation required
- 100X concentration
- 90% yield
- Lenti-X Concentrator can also concentrate MMLV and MSCV retrovirus supernatants utilizing the longer incubation times recommended in the Retro-X Concentrator Protocol-At-A-Glance
Applications
- Lentiviral transduction studies
- Increased multiplicity of infection (MOI)
- Rescue of low-titer supernatants
Rapid lentivirus concentration
Rapid lentivirus concentration. Add Lenti-X Concentrator reagent to clarified viral supernatant, incubate for 30 min to overnight at 4°C, and spin. That’s it.
Lentiviral concentration with high yield
Lentiviral concentration with high yield. Lentiviral supernatant from a pLVX-ZsGreen1 vector was concentrated from 3 ml down to 30 µl using the Lenti-X Concentrator Reagent. The titer before and after concentration were measured and confirmed that >90% of the lentivirus remained functional. Samples were titrated on HT1080 cells and analyzed by flow cytometry 48 hr post-transduction.
Concentrate lentivirus from any volume or from any lentiviral titer
Concentrate lentivirus from any volume or from any lentiviral titer. Lentiviral supernatant was diluted into 250 ml and then concentrated down to 2.5 ml using Lenti-X Concentrator (Panel A). 10-fold serial dilutions of a high-titer lentiviral supernatant (high, medium, and low) were concentrated from a volume of 10 ml down to 100 µl using the Lenti-X Concentrator Reagent (Panel B). Titrations were performed using HT1080 cells and flow cytometry 48 hr post-transduction.
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Rapid lentivirus concentration
Rapid lentivirus concentration. Add Lenti-X Concentrator reagent to clarified viral supernatant, incubate for 30 min to overnight at 4°C, and spin. That’s it.
Lentiviral concentration with high yield
Lentiviral concentration with high yield. Lentiviral supernatant from a pLVX-ZsGreen1 vector was concentrated from 3 ml down to 30 µl using the Lenti-X Concentrator Reagent. The titer before and after concentration were measured and confirmed that >90% of the lentivirus remained functional. Samples were titrated on HT1080 cells and analyzed by flow cytometry 48 hr post-transduction.
Concentrate lentivirus from any volume or from any lentiviral titer
Concentrate lentivirus from any volume or from any lentiviral titer. Lentiviral supernatant was diluted into 250 ml and then concentrated down to 2.5 ml using Lenti-X Concentrator (Panel A). 10-fold serial dilutions of a high-titer lentiviral supernatant (high, medium, and low) were concentrated from a volume of 10 ml down to 100 µl using the Lenti-X Concentrator Reagent (Panel B). Titrations were performed using HT1080 cells and flow cytometry 48 hr post-transduction.
