Lambda DNA
Lambda DNA
Lambda DNA, a linear, double-stranded phage DNA containing 12 bp single-stranded complementary 5'-ends, is derived from an Escherichia coli bacteriophage (Bacteriophage lambda cI857 Sam7). Lambda DNA (48,502 bp) may be used as a molecular weight size marker during nucleic acid gel analysis following digestion with a restriction enzyme (such as HindIII). Lambda DNA can also be used as a substrate in restriction enzyme activity assays.
Storage
–20°C
Form
10 mM Tris-HCl (pH 8.0), 1 mM EDTA
Concentration
0.35 μg/μl
GenBank
Entry name: LAMBDA, Accession Nos.: V00636; J02459, M17233, X00906
Product citations
Goldberg, A. R. & Howe, M. New mutations in the S cistron of bacteriophage lambda affecting host cell lysis. Virology 38, 200–2 (1969).
Sanger, F., Coulson, A. R., Hong, G. F., Hill, D. F. & Petersen, G. B. Nucleotide sequence of bacteriophage lambda DNA. J. Mol. Biol. 162, 729–73 (1982).
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