LacZ Vectors
Beta-galactosidase is the product of the LacZ gene. We offer three beta-gal mammalian reporter vectors containing the LacZ gene. The vectors provide maximal flexibility in analyzing the effects of cis-regulatory elements on gene expression. They can also be used to normalize transfection efficiencies.
- pBeta-Gal-Basic (sold separately as pLacZ-Basic) lacks eukaryotic promoter and enhancer sequences and can serve as a negative control or to clone promoters for characterization.
- pBeta-Gal-Control (sold separately as pLacZ-Control) contains the SV40 promoter and enhancer sequences and can be used as a positive control or as a reference for comparing the activities of promoters and enhancers.
- pCMV-LacZ is a constitutive mammalian reporter vector which expresses extremely high levels of beta-galactosidase from the human cytomegalovirus immediate early promoter (PCMV IE) and can be used as a reference or control plasmid. Beta-galactosidase activity can be detected with the Luminescent Beta-galactosidase Detection Kit II.
Overview
- LacZ vectors for transfection and normalization controls
- Fast, simple colorimetric and chemiluminescent beta-galactosidase assays
- Complete system includes vectors plus the chemiluminescent beta-galactosidase assay
Applications
- Reporter gene assays with the beta-galactosidase reporter
- Monitoring transfection efficiencies & normalizing transfections
- Promoter/enhancer studies
Flow chart of a chemiluminescent beta-galactosidase assay
Flow chart of a chemiluminescent beta-galactosidase assay.
The pBeta gal mammalian reporter vectors
The pBeta gal mammalian reporter vectors.
The pCMV Beta mammalian reporter vector
The pCMV Beta mammalian reporter vector.
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Flow chart of a chemiluminescent beta-galactosidase assay
Flow chart of a chemiluminescent beta-galactosidase assay.
The pBeta gal mammalian reporter vectors
The pBeta gal mammalian reporter vectors.
The pCMV Beta mammalian reporter vector
The pCMV Beta mammalian reporter vector.
