LA Taq PCR kit
LA Taq PCR kit
The LA PCR Kit, Version 2.1 contains all the reagents needed for amplification of long DNA templates, enabling routine amplification of products up to 20 kb in length, including GC-rich amplicons. For some DNA templates, amplification of up to 48 kb is possible. This long-range PCR kit contains TaKaRa LA Taq DNA Polymerase, buffers, MgCl2, dNTPs, molecular weight markers, and control templates, plus corresponding primers to ensure optimal PCR performance during long PCR.
Overview
- Genomic DNA template amplification up to 30 kb, with included control reactions
- Lambda DNA template amplification up to 48 kb, with included control reactions
- Longer and more accurate PCR products
- Specialized 2X GC buffer provided for templates with high GC content or extensive secondary structure
PCR products
PCR products generated with LA Taq DNA Polymerase contain a mixture of 3'-A overhangs and blunt ends, resulting in >80% cloning efficiency in T-vectors. However, the efficiency of cloning longer products (>5 kb) into T-vectors is quite low.
Amplification of human genomic DNA with Takara LA Taq DNA Polymerase
Amplification of human genomic DNA with Takara LA Taq DNA Polymerase. Purified human genomic DNA (500 ng in a 50 µL reaction) was used as a template for PCR amplification of regions of the beta-globin locus and the TPA gene with Takara LA Taq polymerase. The sizes of the amplified products were 17.5 kb (lane 1), 21.5 kb (lane 2), and 27 kb (lane 3). Lane M contains High MW Markers (Fisher Scientific).
Amplification of E. coli genomic DNA regions with Takara LA Taq polymerase.
Amplification of E. coli genomic DNA regions with Takara LA Taq polymerase. DNA fragments up to 38 kb were successfully amplified with the LA PCR Kit Version 2.1, facilitating studies using P1 and cosmid clones.
Write Your Own Review
Amplification of human genomic DNA with Takara LA Taq DNA Polymerase
Amplification of human genomic DNA with Takara LA Taq DNA Polymerase. Purified human genomic DNA (500 ng in a 50 µL reaction) was used as a template for PCR amplification of regions of the beta-globin locus and the TPA gene with Takara LA Taq polymerase. The sizes of the amplified products were 17.5 kb (lane 1), 21.5 kb (lane 2), and 27 kb (lane 3). Lane M contains High MW Markers (Fisher Scientific).
Amplification of E. coli genomic DNA regions with Takara LA Taq polymerase.
Amplification of E. coli genomic DNA regions with Takara LA Taq polymerase. DNA fragments up to 38 kb were successfully amplified with the LA PCR Kit Version 2.1, facilitating studies using P1 and cosmid clones.
