LA Taq DNA polymerase—for long-range PCR

TaKaRa LA Taq DNA Polymerase combines Taq DNA polymerase and a DNA proofreading polymerase, with 3’→5’ exonuclease activity, to enable PCR amplification of very long DNA templates (long-range PCR). This mixture of enzymes allows for long and accurate (LA) PCR of targets from a variety of templates, including genomic DNA. LA Taq DNA polymerase is supplied with optimized LA PCR Buffer II (with or without Mg2+) and dNTPs.

The presence of the proofreading polymerase increases fidelity as compared to Taq polymerase alone. Using the LA Taq long-PCR system, routine extensions of 20 kb are possible, and products of up to 48 kb can be obtained for some templates.

Overview

• Robust amplification of long DNA templates (long-range PCR)—products up to 48 kb are possible
• Specially optimized buffer (LA Buffer II)—less optimization is required and gives greater yield
• DNA proofreading polymerase mix—offers higher fidelity compared with conventional Taq DNA polymerase

Applications

• Long-range PCR to amplify products up to 48 kb
• Amplification of highly homologous sequences
• Pseudogene identification
• Mitochondrial DNA sequencing

PCR products

PCR products generated with LA Taq contain a mixture of 3'-A overhangs and blunt ends which allows >80% cloning efficiency into T-vectors. However, the efficiency of cloning longer products (>5 kb) into T-vectors is quite low.