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KpnI
KpnI restriction site:
G GTAC | C
C | CATG G
Supplied buffer: L
Effect of DNA methylation: enzyme activity is not affected by CG methylase
Reaction temperature: 37°C
- Source: Klebsiella pneumonia
- Concentration: 4–12 units/µl [High concentration: 30–60 units/µl]
- Substrate for unit definition: lambda DNA
- Star activity: unrelated site may be cut in the presence of DMSO
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
KpnI restriction site:
G GTAC | C
C | CATG G
Supplied buffer: L
Effect of DNA methylation: enzyme activity is not affected by CG methylase
Reaction temperature: 37°C
- Source: Klebsiella pneumonia
- Concentration: 4–12 units/µl [High concentration: 30–60 units/µl]
- Substrate for unit definition: lambda DNA
- Star activity: unrelated site may be cut in the presence of DMSO
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
