Kanamycin Resistant pUC Vectors
Kanamycin Resistant pUC Vectors
Brand: Takara Bio.
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Kanamycin Resistant pUC Vectors
Kanamycin resistant pUC vectors
The kanamycin resistant pUC vectors pHSG298 and pHSG299 contain the kanamycin resistance gene, the pMB1-derived origin of replication (ori), and the beta-galactosidase coding gene lacZ. These kanamycin resistant pUC vectors also contain a pUC19-derived multiple cloning site (MCS) within the lacZ gene, enabling recombinant clones to be verified through culture plates containing IPTG and X-Gal. High target gene expression is enabled by the presence of the lac promoter in both the pHSG298 and pHSG299 vectors.
Both pHSG298 and pHSG299 can be analyzed via dideoxy DNA sequencing using an M13 primer, and large recombinant chloramphenicol resistant pUC vectors may be analyzed using the Deletion Kit for Kilo-Sequencing (Cat. # 6030).
Applications
- lac promoter-enabled target gene cloning and expression
- DNA sequencing using an M13 primer
- Long DNA sequencing using the Deletion Kit for Kilo-Sequencing
Purity
- >70% double-stranded, covalently closed circular form (RF1) DNA
- Multiple cloning site sequence-verified via dideoxy DNA sequencing
Storage
–20°C
Notes
The pHSG299 DNA (Cat.# 3299) sequence differs from the sequence of pHSG299 registered with GenBank at the following positions:
1663 C → deletion
1808 C → T
2228–2229 AT → TA
2474 G → A
2667–2668 GA → deletion
Concentration
250–1,000 µg/ml
Chain Length
- pHSG298: 2,675 bp
- pHSG299: 2,673 bp
GenBank
* Except for the MCS, pHSG298 has the same general sequence as pHSG299 with the following exception: pHSG298 lacks a G at position 1,446 in the sequence of pHSG299 that is registered with GenBank.
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Purity
- >70% double-stranded, covalently closed circular form (RF1) DNA
- Multiple cloning site sequence-verified via dideoxy DNA sequencing
Storage
–20°C
Notes
The pHSG299 DNA (Cat.# 3299) sequence differs from the sequence of pHSG299 registered with GenBank at the following positions:
1663 C → deletion
1808 C → T
2228–2229 AT → TA
2474 G → A
2667–2668 GA → deletion
Concentration
250–1,000 µg/ml
Chain Length
- pHSG298: 2,675 bp
- pHSG299: 2,673 bp
GenBank
* Except for the MCS, pHSG298 has the same general sequence as pHSG299 with the following exception: pHSG298 lacks a G at position 1,446 in the sequence of pHSG299 that is registered with GenBank.
