iPSC Single-Cell Cloning Culture Media Kit
iPSC Single-Cell Cloning Culture Media Kit
Brand: Takara Bio.
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iPSC Single-Cell Cloning Culture Media Kit
The Cellartis iPSC Single-Cell Cloning DEF-CS Culture Media Kit (Cat. # Y30021). This is a complete system for efficient expansion and scale-up manufacturing of hiPS cells in a feeder-free and defined environment. The kit contains all the necessary reagents to go from seeding single hiPS cells into 96-well plates to expansion of clones into 48-well plates. Following clonal expansion, hiPS cells can continue to be cultured using the Cellartis DEF-CS 500 Culture System (Cat. # Y30010).
Overview
- Highly efficient gene editing in hiPS cells—use either electroporation or gesicles to deliver Cas9 protein and sgRNA with no genomic integration and reduced off-target effects
- Superior single-cell survival—edited hiPS cells exhibit high (typically ~50%) survival when seeded as single cells in a 96-well plate
- Maintenance of pluripotency after editing and during single-cell cloning—hiPS cells maintain high levels (>90%) of pluripotency markers Oct-4, TRA-1-60, and SSEA-4
- Stable karyotype from start to finish—hiPS cells maintain a normal and stable karyotype throughout editing, single-cell cloning, and expansion
- Flexibility to perform gene editing experiments your way—choose from complete kits that provide editing and single-cell cloning solutions using either electroporation-based (Cat. # 632643) or gesicle-based (Cat. # 632642) CRISPR/Cas9 techniques, or utilize the culture media kit (Cat. # Y30021) to perform efficient single-cell cloning following your own editing experiments
- Continuous use of DEF-CS technology throughout gene editing/single-cell cloning experiments—use the Cellartis DEF-CS 500 Culture System (Cat. # Y30010) before and during gene editing experiments, then during scale-up after single-cell cloning experiments
Applications
- CRISPR/Cas9-mediated gene editing of hiPSCs
- Single-cell cloning of hiPSCs
- Disease modeling
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