In-Fusion SMARTer Directional cDNA Library Construction Kit
In-Fusion SMARTer Directional cDNA Library Construction Kit
Brand: Takara Bio.
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In-Fusion SMARTer Directional cDNA Library Construction Kit
The In-Fusion SMARTer Directional cDNA Library Construction Kit provides a sensitive and efficient method for producing high-quality, full-length cDNA libraries. The kit utilizes two of our most innovative technologies: SMART (Switching Mechanism At 5' end of RNA Transcript) technology coupled with PCR amplification makes it possible to generate high yields of full-length double-stranded cDNA from nanograms of poly A+ or total RNA. In-Fusion cloning technology makes it easy to clone your SMARTer cDNA library into any location within any vector. You may use the pSMART2IFD Linearized Vector included in the kit, or any vector of your choice, for cloning your library. Isolated clones from finished libraries can be transferred directly to any linearized expression vector for functional analysis-without the need for compatible restriction sites.
Overview
SMART cDNA Library Construction Kit (Cat. # 634901)
- Generate cDNA from as little as 50 ng of total RNA
- Enrich for full-length cDNA
- Every cDNA inserted into the MCS is expressed in three reading frames
- Utilizes rare SfiI sites to ensure your cDNA is intact
- No adapter ligation is required for cDNA synthesis or cloning
In-Fusion SMARTer Directional cDNA Library Construction Kit (Cat. # 634933)
- Generate high-quality cDNA libraries from as little as 10 ng of total RNA
- Enrich for full-length cDNA
- No restriction enzyme digestion, no blunt-end polishing, and no adapter ligation required for cDNA synthesis and cloning steps
- Insert your library into any expression vector
Applications
- Large-insert cDNA library construction
- Full-length cDNA amplification
- Directional cDNA cloning, adapter-free
- Sequencing
SMART(er) cDNA synthesis compared to conventional cDNA synthesis
SMART(er) cDNA synthesis compared to conventional cDNA synthesis. Unlike conventional cDNA synthesis methods, which involve a multiple enzyme/multiple step procedure, the SMART(er) cDNA synthesis protocol is performed by one reverse transcription reaction, in a single tube, with no adaptor ligation or intervening purification steps. Following PCR amplification, SMART(er) cDNA is immediately available for a variety of downstream applications.
The In-Fusion SMARTer Directional cDNA Library Construction Kit includes components for cDNA synthesis, library construction, and library amplification
The In-Fusion SMARTer Directional cDNA Library Construction Kit includes components for cDNA synthesis, library construction, and library amplification.
Insert size screening by PCR
Insert size screening by PCR. An In-Fusion SMARTer Directional cDNA library was generated from 10 ng of Control Mouse Liver Total RNA. SMARTer ds cDNA was synthesized, size-fractionated, and then cloned into pSMART2IFD using the In-Fusion Cloning Kit. 15 randomly selected colonies from the unamplified library were size screened by PCR according to the protocol in the User Manual. 3-μl samples of PCR products were electrophoresed on a 1.2% agarose/EtBr gel. Lane M: 1-kb DNA ladder.
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SMART(er) cDNA synthesis compared to conventional cDNA synthesis
SMART(er) cDNA synthesis compared to conventional cDNA synthesis. Unlike conventional cDNA synthesis methods, which involve a multiple enzyme/multiple step procedure, the SMART(er) cDNA synthesis protocol is performed by one reverse transcription reaction, in a single tube, with no adaptor ligation or intervening purification steps. Following PCR amplification, SMART(er) cDNA is immediately available for a variety of downstream applications.
The In-Fusion SMARTer Directional cDNA Library Construction Kit includes components for cDNA synthesis, library construction, and library amplification
The In-Fusion SMARTer Directional cDNA Library Construction Kit includes components for cDNA synthesis, library construction, and library amplification.
Insert size screening by PCR
Insert size screening by PCR. An In-Fusion SMARTer Directional cDNA library was generated from 10 ng of Control Mouse Liver Total RNA. SMARTer ds cDNA was synthesized, size-fractionated, and then cloned into pSMART2IFD using the In-Fusion Cloning Kit. 15 randomly selected colonies from the unamplified library were size screened by PCR according to the protocol in the User Manual. 3-μl samples of PCR products were electrophoresed on a 1.2% agarose/EtBr gel. Lane M: 1-kb DNA ladder.
