iDimerize Inducible Heterodimer System
iDimerize Inducible Heterodimer System
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iDimerize Inducible Heterodimer System
The iDimerize Inducible Heterodimer System lets you control the heterodimerization of two different proteins of interest in live cells via a membrane-permeant compound. The system includes the iDimerize Inducible Heterodimer Vector Set 1, which contains three mammalian expression vectors encoding fusion tags that can be easily added to your proteins of interest. The activity and localization of the resulting chimeric proteins can be controlled by the addition of a small molecule to the cell medium. The system also includes the membrane-permeant compound, A/C Heterodimerizer, required for heterodimerization, and two linear selection markers for hygromycin and puromycin resistance.
The iDimerize Inducible Heterodimer System (with Tet-On 3G) lets you control the expression levels and heterodimerization of two different proteins of interest, encoded on a single vector, in live cells. The system includes the iDimerize Inducible Heterodimer Vector Set 2, which contains a mammalian bidirectional expression vector (pTRE3G-BI-Het1) that allows simultaneous, doxycycline-dependent expression of two proteins of interest, tagged with the DmrA and DmrC domains, respectively, in Tet-On 3G transactivator-expressing cells. The activity and localization of the resulting DmrA- and DmrC-tagged proteins is controlled by the addition of the A/C Heterodimerizer.
The iDimerize Inducible Heterodimer System (with Tet-On 3G) includes vectors and linear selection markers, our highly efficient transfection reagent, Xfect, Tet System Approved FBS, and the A/C Heterodimerizer.
Overview
- Achieve small-molecule control of cellular events that are usually activated by protein heterodimerization
- Control the subcellular localization of a target protein
- A/C Heterodimerizer is identical to the AP21967 ligand
- For inducible control of transcription factors, use the iDimerize Regulated Transcription System
- Plasmid and lentiviral (Lenti-X) vector formats are available
- Previously available from ARIAD Pharmaceuticals, Inc., as the ARGENT Regulated Heterodimerization Kit
Applications
- Create and control interactions between two different proteins
- Create conditional alleles of receptors and signaling molecules
- Activate cell surface receptors or other signaling proteins
- Recruit signaling proteins to specific subcellular locations (e.g., the plasma membrane or nucleus)
- Reconstitute enzyme activity by inducing the interaction of separate polypeptides
- Functional analysis of a signaling pathway in multiple cell types
- Inducible protein dimerization in animal models and cultured cell lines
How does iDimerize technology work?
How does iDimerize technology work? A chemical inducer of dimerization, or “dimerizer”, is a cell-permeant organic small molecule with two separate motifs that each bind with high affinity to a specific protein module (Dmr domain) fused onto the protein(s) of interest. Addition of the dimerizer brings the chimeric protein subunits into very close proximity to each other, mimicking the activation of the cellular event that dimerization of interest controls. B/B Homodimerizer induces self-association of two copies of the same protein whereas A/C Heterodimerizer induces association of two different proteins. Conversely, a reverse dimerizer ligand (D/D Solubilizer) will bind to and dissociate a protein that aggregates in its absence.
Inducible dimerization of heterodimers
Inducible dimerization of heterodimers. Fusion proteins are created which contain the DmrA (green) and DmrC (red) dimerization domains respectively. The two proteins do not interact until the A/C heterodimerizer (AP21967) is added. This cell-permeant ligand induces the fusion proteins to interact, which activates downstream events such as signal transduction pathways.
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How does iDimerize technology work?
How does iDimerize technology work? A chemical inducer of dimerization, or “dimerizer”, is a cell-permeant organic small molecule with two separate motifs that each bind with high affinity to a specific protein module (Dmr domain) fused onto the protein(s) of interest. Addition of the dimerizer brings the chimeric protein subunits into very close proximity to each other, mimicking the activation of the cellular event that dimerization of interest controls. B/B Homodimerizer induces self-association of two copies of the same protein whereas A/C Heterodimerizer induces association of two different proteins. Conversely, a reverse dimerizer ligand (D/D Solubilizer) will bind to and dissociate a protein that aggregates in its absence.
Inducible dimerization of heterodimers
Inducible dimerization of heterodimers. Fusion proteins are created which contain the DmrA (green) and DmrC (red) dimerization domains respectively. The two proteins do not interact until the A/C heterodimerizer (AP21967) is added. This cell-permeant ligand induces the fusion proteins to interact, which activates downstream events such as signal transduction pathways.
