Human Universal QUICK-Clone II

Human Universal QUICK-Clone II

Brand: Takara Bio.
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Human Universal QUICK-Clone II
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Human Universal QUICK-Clone™ II
SKU: 637260
2 x 10 Rxns
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Human Universal QUICK-Clone II
Human Universal QUICK-Clone II

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High-purity, double-stranded cDNA for rapid cloning, sequencing, or probe generation. cDNA was synthesized using an oligo(dT) primer and purified to remove interfering RNA. The cDNA was generated from premium RNA prepared from >30 different human tissues.

Overview

  • Highly purified double-stranded cDNA
  • Clone genes directly by PCR, rather than library screening
  • Prepared from high-quality human tissues and cell lines
  • Ideal for amplifying previously isolated, structurally related, or cross-species cDNAs

Applications

  • Clone cDNAs without library screening
  • Generate hybridization probes using gene-specific or degenerate primers
  • Ideal for amplifying previously isolated, structurally related, or cross-species cDNAs

PCR amplification of the full coding region from human TFR mRNA

PCR amplification of the full coding region from human TFR mRNA

PCR amplification of the full coding region from human TFR mRNA. PCR-amplified QUICK-Clone Human Heart cDNA (Cat. # 637213) was digested with restriction enzymes to verify amplification of the complete coding region of the human TFR gene. Lane 1: no restriction enzyme. Lane 2: Hind III. Lane 3: Hpa I. Lane M1: λ/Hind III DNA size marker. Lane M2: ΦX174/Hae III DNA size marker.

QUICK-Clone II Human Universal cDNA is an optimized mixture of over 30 QUICK-Clone cDNAs from normal human tissues

QUICK-Clone II Human Universal cDNA is an optimized mixture of over 30 QUICK-Clone cDNAs from normal human tissues

QUICK-Clone II Human Universal cDNA is an optimized mixture of over 30 QUICK-Clone cDNAs from normal human tissues. (The exact number may vary based on tissue availability.) It has been specially formulated for the amplification of full-length cDNAs representing the majority of human genes.

Lee, C. et al. Generation of cDNA probes directed by amino acid sequence: cloning of urate oxidase. Science 239 (1988).

Parmentier, M. et al. Molecular cloning of the thyrotropin receptor. Science 246 (1989).

Schuchman, E. H., Jackson, C. E. & Desnick, R. J. Human arylsulfatase B: MOPAC cloning, nucleotide sequence of a full-length cDNA, and regions of amino acid identity with arylsulfatases A and C. Genomics 6, 149–158 (1990).

Vallins, W. J. et al. Molecular cloning of human cardiac troponin I using polymerase chain reaction. FEBS Lett. 270, 57–61 (1990).

Wilks, A. F., Kurban, R. R., Hovens, C. M. & Ralph, S. J. The application of the polymerase chain reaction to cloning members of the protein tyrosine kinase family. Gene 85, 67–74 (1989).

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High-purity, double-stranded cDNA for rapid cloning, sequencing, or probe generation. cDNA was synthesized using an oligo(dT) primer and purified to remove interfering RNA. The cDNA was generated from premium RNA prepared from >30 different human tissues.

Overview

  • Highly purified double-stranded cDNA
  • Clone genes directly by PCR, rather than library screening
  • Prepared from high-quality human tissues and cell lines
  • Ideal for amplifying previously isolated, structurally related, or cross-species cDNAs

Applications

  • Clone cDNAs without library screening
  • Generate hybridization probes using gene-specific or degenerate primers
  • Ideal for amplifying previously isolated, structurally related, or cross-species cDNAs

PCR amplification of the full coding region from human TFR mRNA

PCR amplification of the full coding region from human TFR mRNA

PCR amplification of the full coding region from human TFR mRNA. PCR-amplified QUICK-Clone Human Heart cDNA (Cat. # 637213) was digested with restriction enzymes to verify amplification of the complete coding region of the human TFR gene. Lane 1: no restriction enzyme. Lane 2: Hind III. Lane 3: Hpa I. Lane M1: λ/Hind III DNA size marker. Lane M2: ΦX174/Hae III DNA size marker.

QUICK-Clone II Human Universal cDNA is an optimized mixture of over 30 QUICK-Clone cDNAs from normal human tissues

QUICK-Clone II Human Universal cDNA is an optimized mixture of over 30 QUICK-Clone cDNAs from normal human tissues

QUICK-Clone II Human Universal cDNA is an optimized mixture of over 30 QUICK-Clone cDNAs from normal human tissues. (The exact number may vary based on tissue availability.) It has been specially formulated for the amplification of full-length cDNAs representing the majority of human genes.

Lee, C. et al. Generation of cDNA probes directed by amino acid sequence: cloning of urate oxidase. Science 239 (1988).

Parmentier, M. et al. Molecular cloning of the thyrotropin receptor. Science 246 (1989).

Schuchman, E. H., Jackson, C. E. & Desnick, R. J. Human arylsulfatase B: MOPAC cloning, nucleotide sequence of a full-length cDNA, and regions of amino acid identity with arylsulfatases A and C. Genomics 6, 149–158 (1990).

Vallins, W. J. et al. Molecular cloning of human cardiac troponin I using polymerase chain reaction. FEBS Lett. 270, 57–61 (1990).

Wilks, A. F., Kurban, R. R., Hovens, C. M. & Ralph, S. J. The application of the polymerase chain reaction to cloning members of the protein tyrosine kinase family. Gene 85, 67–74 (1989).

Write Your Own Review
You're reviewing:Human Universal QUICK-Clone II
Your Rating