Human Stem Cell-derived Intestinal Epithelial Cells
Human Stem Cell-derived Intestinal Epithelial Cells
Brand: Takara Bio.
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Human Stem Cell-derived Intestinal Epithelial Cells
Cellartis Intestinal Epithelial Cells (from ChiPSC18) are small intestinal epithelial cells derived from human induced pluripotent stem (hiPS) cells. This product includes the following items: one frozen vial of Cellartis Intestinal Epithelial Cells (from ChiPSC18) and one Cellartis IEC Maturation Kit.
Overview
- Cells form tight junctions within 5 days of thawing and can be used on Days 5–8 post-thaw
- Cells express intestinal markers (e.g., villin and CDX2) at high levels
- Drug metabolizing enzyme CYP3A4 and drug transporter PEPT1 are expressed at high levels compared to Caco-2 cells
- Cells form a functional, permeable barrier that can be used to predict in vivo drug permeability and absorption
Applications
- Evaluation of drug absorption and metabolism in the small intestine
- ADME profiling in drug discovery
- Drug permeability assays
Expression of the intestinal epithelial markers villin and CDX2
Intestinal epithelial markers villin and CDX2 are expressed at high levels in our hiPSC-derived intestinal epithelial cells (IEC) compared to Caco-2 cells on Day 7 post-thaw.
Immunostaining of tight junction protein ZO-1
Immunostaining for tight junction protein ZO-1 shows formation of intercellular tight junctions (i.e., zonula occludens) by Day 5 post-thaw.
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Expression of the intestinal epithelial markers villin and CDX2
Intestinal epithelial markers villin and CDX2 are expressed at high levels in our hiPSC-derived intestinal epithelial cells (IEC) compared to Caco-2 cells on Day 7 post-thaw.
Immunostaining of tight junction protein ZO-1
Immunostaining for tight junction protein ZO-1 shows formation of intercellular tight junctions (i.e., zonula occludens) by Day 5 post-thaw.
