Human Mitochondrial DNA (mtDNA) Monitoring Primer Set is designed to quantify the relative number of copies of human mitochondrial DNA (mtDNA) by real-time PCR, using nuclear DNA (nDNA) content as a standard. This set contains primer pairs for the amplification of four regions: two primer pairs for the detection of mtDNA and two primer pairs for the detection of nDNA. Each primer is specifically designed and will not amplify pseudogenes. As the primers are human-specific and will not amplify DNA from mice, human mtDNA content can be monitored in iPS cells or other cells cultured with feeder cells of mouse origin. Using the four primer pairs in this set for real-time PCR, relative quantification of mtDNA is determined from the difference in Ct values for mtDNA and nDNA. This product provides a simple method for monitoring changes in the mtDNA copy number as mtDNA has been reported to increase during differentiation of pluripotent cells, such as iPS cells, into hepatic cells. The primer pairs in this set are compatible with Terra qPCR Direct TB Green Premix (Cat. # 638319) and TB Green Premix Ex Taq II (Tli RNase H Plus) (Cat. # RR820A/B).

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