Human Leukocyte Marathon-Ready cDNA
Human Leukocyte Marathon-Ready cDNA
Brand: Takara Bio.
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Human Leukocyte Marathon-Ready cDNA
Marathon-Ready cDNA is high-quality, double-stranded cDNA which has been ligated to the Marathon Adaptor and is ready for use as a template in 5' and 3' Marathon RACE reactions. In many cases, the full-length cDNA can then be obtained by end-to-end amplification or standard cloning. Enough material is provided for 30 50-μl Marathon RACE reactions.
Overview
- Adaptor-ligated and ready for RACE PCR
- Tissue-specific "pool" of double-stranded cDNA
- Obtain full-length cDNAs without ever constructing or screening a cDNA library
- Perfect template for Marathon cDNA Amplification
Applications
- Rapid amplification of cDNA ends (RACE)
- Study tissue-specific gene expression
- Discover polymorphic forms of mRNA
- Obtain full-length copies of published cDNAs
- Characterization of tissue-specific patterns of gene expression
Generation of full-length cDNA by end-to-end amplification from adaptor-ligated ds cDNA
Generation of full-length cDNA by end-to-end amplification from adaptor-ligated ds cDNA. RACE PCR was performed. The template for Lanes 1–3 was Marathon-Ready Human Placenta cDNA (Cat. # 639311); the template for Lanes 4 & 5 was Marathon-Ready Human Skeletal Muscle cDNA (Cat. # 639313). Lane 1: 5'-RACE product for TFR cDNA. Lane 2: 3'-RACE product for TFR cDNA. Lane 3: full-length TFR cDNA (5.1 kb) generated by end-to-end amplification using 5' and 3' TFR primers. Lane 4: nearly full-length IGFR2 cDNA (8.8 kb) amplified with 5' IGFR2 and 3' IGFR2 primers. Lane 5: full-length IGFR1 cDNA (5.0 kb) amplified with primers 5'-IGFR and 3'-IGFR. Lane M: 1-kb DNA size ladder.
Marathon RACE products obtained using G3PDH primers to amplify Marathon-Ready Human, Mouse, and Rat Heart cDNAs
Marathon RACE products obtained using G3PDH primers to amplify Marathon-Ready Human, Mouse, and Rat Heart cDNAs. RACE PCR was performed using the AP1 primer and the positive control G3PDH primers. The templates used were Marathon-Ready Human Heart (Cat. # 639304), Mouse Heart (Cat. # 639404), and Rat Heart (Cat. # 639416) cDNAs. Lane M: 1-kb DNA size ladder.
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Generation of full-length cDNA by end-to-end amplification from adaptor-ligated ds cDNA
Generation of full-length cDNA by end-to-end amplification from adaptor-ligated ds cDNA. RACE PCR was performed. The template for Lanes 1–3 was Marathon-Ready Human Placenta cDNA (Cat. # 639311); the template for Lanes 4 & 5 was Marathon-Ready Human Skeletal Muscle cDNA (Cat. # 639313). Lane 1: 5'-RACE product for TFR cDNA. Lane 2: 3'-RACE product for TFR cDNA. Lane 3: full-length TFR cDNA (5.1 kb) generated by end-to-end amplification using 5' and 3' TFR primers. Lane 4: nearly full-length IGFR2 cDNA (8.8 kb) amplified with 5' IGFR2 and 3' IGFR2 primers. Lane 5: full-length IGFR1 cDNA (5.0 kb) amplified with primers 5'-IGFR and 3'-IGFR. Lane M: 1-kb DNA size ladder.
Marathon RACE products obtained using G3PDH primers to amplify Marathon-Ready Human, Mouse, and Rat Heart cDNAs
Marathon RACE products obtained using G3PDH primers to amplify Marathon-Ready Human, Mouse, and Rat Heart cDNAs. RACE PCR was performed using the AP1 primer and the positive control G3PDH primers. The templates used were Marathon-Ready Human Heart (Cat. # 639304), Mouse Heart (Cat. # 639404), and Rat Heart (Cat. # 639416) cDNAs. Lane M: 1-kb DNA size ladder.
