Human Bone Marrow QUICK-Clone cDNA
Human Bone Marrow QUICK-Clone cDNA
Brand: Takara Bio.
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Human Bone Marrow QUICK-Clone cDNA
A high-quality cDNA template is necessary to obtain good results from PCR amplification. QUICK-Clone cDNA is premade double-stranded cDNA from which you can amplify sequences of interest using gene-specific primers, and is ideal for amplifying previously isolated, structurally related, or cross-species cDNAs. Synthesized from premium, high-quality poly A+ RNA from human brain and central nervous system tissues using an oligo(dT) primer, QUICK-Clone cDNA is purified to remove residual RNA and size-selected to eliminate cDNA fragments smaller than 400 bp. QUICK-Clone cDNA allows you to amplify cDNAs of interest while avoiding traditional library construction and screening steps, and can also be used to generate hybridization probes using gene-specific or degenerate primers (Lee et al. 1988; Parmentier et al. 1989; Schuchman, Jackson, and Desnick 1990; Vallins et al. 1990; Wilks et al. 1989).
Overview
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Highly purified, double-stranded cDNA
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Clone genes directly by PCR, rather than library screening
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Prepared from high-quality human tissues
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Ideal for amplifying previously isolated, structurally related, or cross-species cDNAs
Applications
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Clone cDNAs without library screening
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Generate hybridization probes using gene-specific or degenerate primers
- Ideal for amplifying previously isolated, structurally related, or cross-species cDNAs
PCR amplification of the full coding region from human TFR mRNA
PCR amplification of the full coding region from human TFR mRNA. PCR-amplified QUICK-Clone Human Heart cDNA (Cat. # 637213) was digested with restriction enzymes to verify amplification of the complete coding region of the human TFR gene. Lane 1: no restriction enzyme. Lane 2: Hind III. Lane 3: Hpa I. Lane M1: λ/Hind III DNA size marker. Lane M2: ΦX174/Hae III DNA size marker.
QUICK-Clone II Human Universal cDNA is an optimized mixture of over 30 QUICK-Clone cDNAs from normal human tissues
QUICK-Clone II Human Universal cDNA is an optimized mixture of over 30 QUICK-Clone cDNAs from normal human tissues. (The exact number may vary based on tissue availability.) It has been specially formulated for the amplification of full-length cDNAs representing the majority of human genes.
Lee, C. C. et al. Generation of cDNA probes directed by amino acid sequence: cloning of urate oxidase. Science 239, 1288–91 (1988).
Parmentier, M. et al. Molecular cloning of the thyrotropin receptor. Science 246, 1620–2 (1989).
Schuchman, E. H., Jackson, C. E. & Desnick, R. J. Human arylsulfatase B: MOPAC cloning, nucleotide sequence of a full-length cDNA, and regions of amino acid identity with arylsulfatases A and C. Genomics 6, 149–58 (1990).
Vallins, W. J. et al. Molecular cloning of human cardiac troponin I using polymerase chain reaction. FEBS Lett. 270, 57–61 (1990).
Wilks, A. F., Kurban, R. R., Hovens, C. M. & Ralph, S. J. The application of the polymerase chain reaction to cloning members of the protein tyrosine kinase family. Gene 85, 67–74 (1989).
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PCR amplification of the full coding region from human TFR mRNA
PCR amplification of the full coding region from human TFR mRNA. PCR-amplified QUICK-Clone Human Heart cDNA (Cat. # 637213) was digested with restriction enzymes to verify amplification of the complete coding region of the human TFR gene. Lane 1: no restriction enzyme. Lane 2: Hind III. Lane 3: Hpa I. Lane M1: λ/Hind III DNA size marker. Lane M2: ΦX174/Hae III DNA size marker.
QUICK-Clone II Human Universal cDNA is an optimized mixture of over 30 QUICK-Clone cDNAs from normal human tissues
QUICK-Clone II Human Universal cDNA is an optimized mixture of over 30 QUICK-Clone cDNAs from normal human tissues. (The exact number may vary based on tissue availability.) It has been specially formulated for the amplification of full-length cDNAs representing the majority of human genes.
