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HL-SAN
This enzyme will allow for removal of nucleic acids in a traditional protein buffer regime – ie the protein is well protected while the nucleic acids are fully removed. Digests DNA and RNA in approximately a 10:1 ratio. Applications in removal of contaminating nucleic acids from purified enzyme/protein preps as well as inline digestion of nucleic acids in protein purifications. The digestion can take place at high salt conditions.
Main Advantages with Salt Active Nuclease
Non specific endonuclease
Optimum activity at high salt concentration (0.5 M NaCl)
Active at low temperatures (20% at 6ºC)
Broad pH range
Temperature stable
A structured workflow for mapping human Sin3 histone deacetylase complex interactions using Halo-MudPIT AP-MS Charles A S Banks, Janet L Thornton, Cassandra G. Eubanks, Mark K Adams, Sayem Miah, Gina Boanca, Xingyu Liu, Maria Katt, Tari Parmely, Laurence A. Florens and Michael P. Washburn. Biochemical Characterization of a Family 15 Carbohydrate Esterase from a Bacterial Marine Arctic Metagenome De Santi C, Willassen NP, Williamson A (2016) Biochemical Characterization of a Family 15 Carbohydrate Esterase from a Bacterial Marine Arctic Metagenome. PLoS ONE 11(7): e0159345. doi:10.1371/journal.pone.0159345 Reducing DNA content during protein purification with an easily removable nuclease Bernd Striberny, Jørn Remi Henriksen, Morten Elde, Olav Lanes, Adele Williamson Recombinant expression and purification of an ATP-dependent DNA ligase from Aliivibrio salmonicida. Williamson A, Pedersen H. (2014) Biochemical characterization of ParI, an orphan C5-DNA methyltransferase from Psychrobacter arcticus 273-4. Grgic, M.; Williamson, A.; Kjaereng Bjerga, G. E.; Altermark, B.; Leiros, I. (2018) Nanopore metagenomics enables rapid clinical diagnosis of bacterial lower respiratory infection Charalampous, T.; Kay, G.L.; Richardson, H.; Aydin, A.; Baldan, R.; Jeanes, C.; Rae, D.; Grundy, S.; Turner, D.J.; Wain, J.; Leggett, R.M.; Livermore, D.M; O’Grady, J. (2019)
This enzyme will allow for removal of nucleic acids in a traditional protein buffer regime – ie the protein is well protected while the nucleic acids are fully removed. Digests DNA and RNA in approximately a 10:1 ratio. Applications in removal of contaminating nucleic acids from purified enzyme/protein preps as well as inline digestion of nucleic acids in protein purifications. The digestion can take place at high salt conditions.
Main Advantages with Salt Active Nuclease
Non specific endonuclease
Optimum activity at high salt concentration (0.5 M NaCl)
Active at low temperatures (20% at 6ºC)
Broad pH range
Temperature stable
A structured workflow for mapping human Sin3 histone deacetylase complex interactions using Halo-MudPIT AP-MS Charles A S Banks, Janet L Thornton, Cassandra G. Eubanks, Mark K Adams, Sayem Miah, Gina Boanca, Xingyu Liu, Maria Katt, Tari Parmely, Laurence A. Florens and Michael P. Washburn. Biochemical Characterization of a Family 15 Carbohydrate Esterase from a Bacterial Marine Arctic Metagenome De Santi C, Willassen NP, Williamson A (2016) Biochemical Characterization of a Family 15 Carbohydrate Esterase from a Bacterial Marine Arctic Metagenome. PLoS ONE 11(7): e0159345. doi:10.1371/journal.pone.0159345 Reducing DNA content during protein purification with an easily removable nuclease Bernd Striberny, Jørn Remi Henriksen, Morten Elde, Olav Lanes, Adele Williamson Recombinant expression and purification of an ATP-dependent DNA ligase from Aliivibrio salmonicida. Williamson A, Pedersen H. (2014) Biochemical characterization of ParI, an orphan C5-DNA methyltransferase from Psychrobacter arcticus 273-4. Grgic, M.; Williamson, A.; Kjaereng Bjerga, G. E.; Altermark, B.; Leiros, I. (2018) Nanopore metagenomics enables rapid clinical diagnosis of bacterial lower respiratory infection Charalampous, T.; Kay, G.L.; Richardson, H.; Aydin, A.; Baldan, R.; Jeanes, C.; Rae, D.; Grundy, S.; Turner, D.J.; Wain, J.; Leggett, R.M.; Livermore, D.M; O’Grady, J. (2019)
