HL-dsDNase is a genetically engineered version of dsDNase that is rapidly and completely inactivated at moderately high temperatures. HL-dsDNase is inactivated at 58°C with a pH above 8.0. This makes this product very useful for removal of DNA from RNA preparations, since the enzyme may be heat inactivated without risk of auto-degradation of RNA in the presence of magnesium.
Main advantages with HL-dsDNase
Double-strand DNA specific endonuclease
Easily heat-inactivated by very moderate heat treatment
The HL-dsDNase can be heat inactivated by heat treatment at 5 min at 58˚C. The enzyme requires 1 mM DTT and pH ≥ 8 for complete inactivation.
Specifications
Unit definition One unit is defined as an increase in absorbance at 260 nm of 0.001 per minute, using 50 µg/ml of high MW DNA in 100 mM Na-acetate pH 5.0 and 5 mM MgCl2.
Specific activity Ca. 200 000 Kunitz Units/mg.
Activity The HL-dsDNase is highly active in a temperature range of 20-40°C. It needs at least 2.5 mM Mg for activity and has an optimal pH at 7.5.
Storage buffer 20 mM Tris-HCl pH 7.5, 2 mM MgCl2, 10 mM NaCl, 0.01% (v/v) Triton X-100, 50% (v/v) glycerol.
Purity HL-dsDNase is purified to apparent homogeneity.
Storage Minimum shelf life is 2 years at -20°C. The enzyme also tolerates multiple freeze-thaw cycles.
HL-dsDNase is a genetically engineered version of dsDNase that is rapidly and completely inactivated at moderately high temperatures. HL-dsDNase is inactivated at 58°C with a pH above 8.0. This makes this product very useful for removal of DNA from RNA preparations, since the enzyme may be heat inactivated without risk of auto-degradation of RNA in the presence of magnesium.
Main advantages with HL-dsDNase
Double-strand DNA specific endonuclease
Easily heat-inactivated by very moderate heat treatment
The HL-dsDNase can be heat inactivated by heat treatment at 5 min at 58˚C. The enzyme requires 1 mM DTT and pH ≥ 8 for complete inactivation.
Specifications
Unit definition One unit is defined as an increase in absorbance at 260 nm of 0.001 per minute, using 50 µg/ml of high MW DNA in 100 mM Na-acetate pH 5.0 and 5 mM MgCl2.
Specific activity Ca. 200 000 Kunitz Units/mg.
Activity The HL-dsDNase is highly active in a temperature range of 20-40°C. It needs at least 2.5 mM Mg for activity and has an optimal pH at 7.5.
Storage buffer 20 mM Tris-HCl pH 7.5, 2 mM MgCl2, 10 mM NaCl, 0.01% (v/v) Triton X-100, 50% (v/v) glycerol.
Purity HL-dsDNase is purified to apparent homogeneity.
Storage Minimum shelf life is 2 years at -20°C. The enzyme also tolerates multiple freeze-thaw cycles.
