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HhaI
HhaI restriction site:
G CG | C
C | GC G
Supplied buffer: M
Effect of DNA methylation: enzyme activity is affected by CG methylase
Reaction temperature: 37°C
- Source: Haemophilus haemolyticus
- Concentration: 4–12 units/µl
- Substrate for unit definition: lambda DNA
- Star activity: unrelated site may be cut in the presence of DMSO or high concentrations of glycerol
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
HhaI restriction site:
G CG | C
C | GC G
Supplied buffer: M
Effect of DNA methylation: enzyme activity is affected by CG methylase
Reaction temperature: 37°C
- Source: Haemophilus haemolyticus
- Concentration: 4–12 units/µl
- Substrate for unit definition: lambda DNA
- Star activity: unrelated site may be cut in the presence of DMSO or high concentrations of glycerol
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
