PrimerArray Hepatic Differentiation (Human) is a set of real-time RT-PCR primers for the analysis of the expression of genes associated with hepatic differentiation. This array contains primer pairs optimized for real-time RT-PCR for the analysis of 88 genes associated with pluripotency, endoderm, and hepatic differentiation and 8 housekeeping genes. When comparing an unknown sample to a control sample, gene expression differences can be expressed using the relative quantification method.
Overview
RT-PCR primers arrayed in a 96-well plate allows rapid assessment of induction and differentiation of pluripotent cells into hepatocytes
Minimizes pipetting and reduces error
Enables high-throughput analysis of agents affecting hepatocyte differentiation
Optimized for superior performance with TB Green Ex Taq qPCR kits
PrimerArray analysis tool makes data analysis easy
Compatible with all standard real-time PCR instrument systems
Applications
Monitoring hepatocyte differentiation/induction
Screening for expression of hepatocyte marker genes in response to conditions or agents affecting differentiation
Procedural overview for hepatocyte differentiation gene expression analysis by real time RT-PCR
The overall workflow for the PrimerArray Series primer sets is straightforward:
RNA extraction (30 min): We recommend using NucleoSpin RNA (Cat. # 740955.50) or RNAiso Plus (Cat. # 9108/9109, not available in all geographic locations). Removal of genomic DNA using Recombinant DNase I (RNase-free) (Cat. # 2270A) is recommended.
Dispense real-time PCR reaction solution (10 min): Prepare a master mix containing the cDNA sample and TB Green Premix Ex Taq II (Tli RNase H Plus) (Cat. # RR820A); dispense into 96-well plate.
Add PrimerArray primers (5 min): Use 4 µl of PrimerArray Series primers per reaction
Real-time PCR (1–2 hours): PrimerArray Series primers are compatible with all commonly used real-time PCR systems
Data analysis (30 minutes): Use the PrimerArray analysis tool
PrimerArray Analysis Tool for Hepatic Differentiation (Human)
The PrimerArray analysis tool facilitates data analysis and can be downloaded here. To access an instruction guide about how to use the tool, click on the Details down arrow icon in the product table, click on the Documents tab, and then expand the drop-down menu under User Manuals.
Information about RT-PCR primers for PrimerArray Hepatic Differentiation (Human)
To download primer information for this set of PrimerArray RT-PCR primers, click on the Details down arrow icon in the product table, click on the Documents tab, and then expand the drop-down menu under User Manuals.
PrimerArray Hepatic Differentiation (Human) is a set of real-time RT-PCR primers for the analysis of the expression of genes associated with hepatic differentiation. This array contains primer pairs optimized for real-time RT-PCR for the analysis of 88 genes associated with pluripotency, endoderm, and hepatic differentiation and 8 housekeeping genes. When comparing an unknown sample to a control sample, gene expression differences can be expressed using the relative quantification method.
Overview
RT-PCR primers arrayed in a 96-well plate allows rapid assessment of induction and differentiation of pluripotent cells into hepatocytes
Minimizes pipetting and reduces error
Enables high-throughput analysis of agents affecting hepatocyte differentiation
Optimized for superior performance with TB Green Ex Taq qPCR kits
PrimerArray analysis tool makes data analysis easy
Compatible with all standard real-time PCR instrument systems
Applications
Monitoring hepatocyte differentiation/induction
Screening for expression of hepatocyte marker genes in response to conditions or agents affecting differentiation
Procedural overview for hepatocyte differentiation gene expression analysis by real time RT-PCR
The overall workflow for the PrimerArray Series primer sets is straightforward:
RNA extraction (30 min): We recommend using NucleoSpin RNA (Cat. # 740955.50) or RNAiso Plus (Cat. # 9108/9109, not available in all geographic locations). Removal of genomic DNA using Recombinant DNase I (RNase-free) (Cat. # 2270A) is recommended.
Dispense real-time PCR reaction solution (10 min): Prepare a master mix containing the cDNA sample and TB Green Premix Ex Taq II (Tli RNase H Plus) (Cat. # RR820A); dispense into 96-well plate.
Add PrimerArray primers (5 min): Use 4 µl of PrimerArray Series primers per reaction
Real-time PCR (1–2 hours): PrimerArray Series primers are compatible with all commonly used real-time PCR systems
Data analysis (30 minutes): Use the PrimerArray analysis tool
PrimerArray Analysis Tool for Hepatic Differentiation (Human)
The PrimerArray analysis tool facilitates data analysis and can be downloaded here. To access an instruction guide about how to use the tool, click on the Details down arrow icon in the product table, click on the Documents tab, and then expand the drop-down menu under User Manuals.
Information about RT-PCR primers for PrimerArray Hepatic Differentiation (Human)
To download primer information for this set of PrimerArray RT-PCR primers, click on the Details down arrow icon in the product table, click on the Documents tab, and then expand the drop-down menu under User Manuals.