Heat&Run gDNA Removal Kit
Heat&Run gDNA Removal Kit
Brand: ArcticZymes
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Heat&Run gDNA Removal Kit
Kit allows rapid, effortless and complete removal of gDNA in RNA preps from any source
The kit contains HL-dsDNase which efficiently removes gDNA from RNA preps before running RT-qPCR.
- gDNA is removed, leaving RNA ready for reverse transcription in the same tube.
- Heat-labile dsDNase can easily be inactivated.
- This procedure minimizes pipetting steps and reduces hands-on time.
- The Heat&Run kit is especially well suited for high throughput experiments.
The high activity of the HL-dsDNase at low temperature and its heat-lability makes it ideal to use in a combination with a heat activated RT enzyme. The contaminating genomic DNA is cleaved at ambient temperature and increasing the temperature over 50°C will inactivate the DNase and start the Reverse Transcriptase.
Kit Contents
- 10X reaction Buffer
- HL-dsDNase (50 reactions)
Storage Conditions: Store at -20˚C.
Sample Material: The Heat&Run kit is suited for gDNA removal of RNA from any source.
Quality Control: The kit is tested for absence of RNase.
- Expressed repetitive elements are broadly applicable reference targets for normalization of reverse transcription-qPCR data in mice
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Genome Announc. May 2017 vol. 5 no. 19 e00310-17 - Increased chromosomal radiosensitivity in asymptomatic carriers of a heterozygous BRCA1 mutationAnnelot Baert, Julie Depuydt, Tom Van Maerken, Bruce Poppe, Fransiska Malfait, Katrien Storm, Jenneke van den Ende, Tim Van Damme, Sylvia De Nobele, Gianpaolo Perletti, Kim De Leeneer, Kathleen B. M. Claes, and Anne Vral (2016)
Breast Cancer Research : BCR 18 (2016): 52. PMC. Web. 1 June 2016.
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