HAT-tag Expression System
HAT-tag Expression System
Brand: Takara Bio.
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HAT-tag Expression System
This is a complete system for bacterial expression and IMAC purification of proteins. The system is based on the pHAT Vectors, which contain a novel histidine affinity tag (HAT) that allows one-step purification of tagged proteins from crude cell lysates. The HAT sequence in these vectors is followed by an enterokinase (EK) cleavage site and an MCS. The HAT
Overview
- Purify proteins at neutral pH
- Use under native or denaturing conditions
- Higher protein solubility
- Obtain optimum purification with TALON IMAC resin
Applications
Easy protein expression and purification in bacterial systems, for higher protein solubility
FPLC purification of HAT-tagged protein with TALON Superflow
FPLC purification of HAT-tagged protein with TALON Superflow. E. coli cells were extracted in 50 mM sodium phosphate, 0.3 M NaCl, 5 mM imidazole, pH 7.0 and eluted in 150 mM imidazole (1). Panel A. Cell lysate was purified with a TALON Superflow Column. Panel B. SDS-PAGE analysis of the procedure. M: molecular weight marker.
pHAT10/11/12 and pHAT20 Vector maps
pHAT10/11/12 and pHAT20 Vector maps.
Amino acid sequences of 6xHis, 6xHN, and HAT polyhistidine tags
Amino acid sequences of 6xHis, 6xHN, and HAT polyhistidine tags.
Features and benefits of the HAT (histidine affinity tag) Protein Expression & Purification System, which allows proteins expressed in bacteria to be purified at neutral or physiological pH and in native or denaturing conditions
Features and benefits of the HAT (histidine affinity tag) Protein Expression & Purification System, which allows proteins expressed in bacteria to be purified at neutral or physiological pH and in native or denaturing conditions.
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FPLC purification of HAT-tagged protein with TALON Superflow
FPLC purification of HAT-tagged protein with TALON Superflow. E. coli cells were extracted in 50 mM sodium phosphate, 0.3 M NaCl, 5 mM imidazole, pH 7.0 and eluted in 150 mM imidazole (1). Panel A. Cell lysate was purified with a TALON Superflow Column. Panel B. SDS-PAGE analysis of the procedure. M: molecular weight marker.
pHAT10/11/12 and pHAT20 Vector maps
pHAT10/11/12 and pHAT20 Vector maps.
Amino acid sequences of 6xHis, 6xHN, and HAT polyhistidine tags
Amino acid sequences of 6xHis, 6xHN, and HAT polyhistidine tags.
Features and benefits of the HAT (histidine affinity tag) Protein Expression & Purification System, which allows proteins expressed in bacteria to be purified at neutral or physiological pH and in native or denaturing conditions
Features and benefits of the HAT (histidine affinity tag) Protein Expression & Purification System, which allows proteins expressed in bacteria to be purified at neutral or physiological pH and in native or denaturing conditions.
