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HapII (HpaII, MspI)
HapII (HpaII, MspI) restriction site:
C | CG G
G GC | C
Supplied buffer: L
Effect of DNA methylation: enzyme activity is affected by CG methylase
Reaction temperature: 37°C
- Source: Haemophilus aphrophilus
- Concentration: 4–12 units/µl [High concentration: 30–60 units/µl]
- Substrate for unit definition: lambda DNA
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
HapII (HpaII, MspI) restriction site:
C | CG G
G GC | C
Supplied buffer: L
Effect of DNA methylation: enzyme activity is affected by CG methylase
Reaction temperature: 37°C
- Source: Haemophilus aphrophilus
- Concentration: 4–12 units/µl [High concentration: 30–60 units/µl]
- Substrate for unit definition: lambda DNA
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
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