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HaeIII
HaeIII restriction site:
GG | CC
CC | GG
Supplied buffer: M
Effect of DNA methylation: enzyme activity is not affected by CG methylase
Reaction temperature: 37°C
- Source: Haemophilus aegyptius
- Concentration: 4–12 units/µl [High concentration: 30–60 units/µl]
- Substrate for unit definition: lambda DNA
- Star activity: unrelated site may be cut in the presence of high concentrations of glycerol
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
HaeIII restriction site:
GG | CC
CC | GG
Supplied buffer: M
Effect of DNA methylation: enzyme activity is not affected by CG methylase
Reaction temperature: 37°C
- Source: Haemophilus aegyptius
- Concentration: 4–12 units/µl [High concentration: 30–60 units/µl]
- Substrate for unit definition: lambda DNA
- Star activity: unrelated site may be cut in the presence of high concentrations of glycerol
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
