Glycoprotein Western Detection Kit
Glycoprotein Western Detection Kit
Brand: Takara Bio.
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Glycoprotein Western Detection Kit
The Glycoprotein Western Detection Kit, an accessory product to the Glycoprotein Enrichment Resin, is designed for selective staining of glycoproteins on Western blots. The kit uses a modification of the periodic acid-Schiff method to stain glycoprotein carbohydrate moieties, yielding colored magenta bands. The periodic acid-Schiff reagent, which stains vicinal diol groups found mainly on peripheral sugars and sialic acids, is commonly used as a general glycoprotein stain.The Glycoprotein Western Detection Kit provides rapid, sensitive, antibody-INDEPENDENT detection of enriched glycoproteins on Western blots.
Overview
- Detection kit provides rapid (1 hr), sensitive, and specific antibody-INDEPENDENT Western detection
Highly sensitive and selective detection of purified glycoproteins with Clontech’s Glycoprotein Western Detection Kit
Highly sensitive and selective detection of purified glycoproteins with Clontech’s Glycoprotein Western Detection Kit. Panel A. Varying amounts of purified horseradish peroxidase (HRP), a known glycoprotein (Lanes 1–4), and soybean trypsin inhibitor (SBTI), a nonglycosylated protein (Lanes 5–8), were electrophoresed on a 4–15% SDS-PAGE gel and transferred to a nitrocellulose membrane. Glycoprotein bands were specifically detected in Lanes 1–4, but not Lanes 5–8, using the Glycoprotein Western Detection Kit. Lane M: molecular weight marker. Lane 1: 700 ng HRP. Lane 2: 350 ng HRP. Lane 3: 140 ng HRP. Lane 4: 70 ng HRP. Lane 5: 700 ng SBTI. Lane 6: 350 ng SBTI. Lane 7: 140 ng SBTI. Lane 4: 70 ng SBTI. Panel B. Varying amounts of three different purified glycoproteins [fetuin (Lanes 1–5), alpha-1 acid glycoprotein (AGP; Lanes 6–10), and horseradish peroxidase (HRP; Lanes 11 & 12)] were electrophoresed on a 4–15% SDS-PAGE gel, transferred to a nitrocellulose membrane, and detected using the Glycoprotein Western Detection Kit, according to the user manual. Lane M: molecular weight marker. Lane 1: 1 μg fetuin. Lane 2: 500 ng fetuin. Lane 3: 200 ng fetuin. Lane 4: 100 ng fetuin. Lane 5: 50 ng fetuin. Lane 6: 1 μg AGP. Lane 7: 500 ng AGP. Lane 8: 200 ng AGP. Lane 9: 100 ng AGP. Lane 10: 50 ng AGP. Lane 11: 350 ng HRP. Lane 12: 140 ng HRP.
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Highly sensitive and selective detection of purified glycoproteins with Clontech’s Glycoprotein Western Detection Kit
Highly sensitive and selective detection of purified glycoproteins with Clontech’s Glycoprotein Western Detection Kit. Panel A. Varying amounts of purified horseradish peroxidase (HRP), a known glycoprotein (Lanes 1–4), and soybean trypsin inhibitor (SBTI), a nonglycosylated protein (Lanes 5–8), were electrophoresed on a 4–15% SDS-PAGE gel and transferred to a nitrocellulose membrane. Glycoprotein bands were specifically detected in Lanes 1–4, but not Lanes 5–8, using the Glycoprotein Western Detection Kit. Lane M: molecular weight marker. Lane 1: 700 ng HRP. Lane 2: 350 ng HRP. Lane 3: 140 ng HRP. Lane 4: 70 ng HRP. Lane 5: 700 ng SBTI. Lane 6: 350 ng SBTI. Lane 7: 140 ng SBTI. Lane 4: 70 ng SBTI. Panel B. Varying amounts of three different purified glycoproteins [fetuin (Lanes 1–5), alpha-1 acid glycoprotein (AGP; Lanes 6–10), and horseradish peroxidase (HRP; Lanes 11 & 12)] were electrophoresed on a 4–15% SDS-PAGE gel, transferred to a nitrocellulose membrane, and detected using the Glycoprotein Western Detection Kit, according to the user manual. Lane M: molecular weight marker. Lane 1: 1 μg fetuin. Lane 2: 500 ng fetuin. Lane 3: 200 ng fetuin. Lane 4: 100 ng fetuin. Lane 5: 50 ng fetuin. Lane 6: 1 μg AGP. Lane 7: 500 ng AGP. Lane 8: 200 ng AGP. Lane 9: 100 ng AGP. Lane 10: 50 ng AGP. Lane 11: 350 ng HRP. Lane 12: 140 ng HRP.
