Rapid affinity purification of GST-tagged proteins

Affinity adsorbent-based immobilized resin based on cross-linked agarose Superflow for the purification of recombinant glutathione S-transferase (GST)-tagged biomolecules under native conditions. This resin permits one-step purification of GST-tagged recombinant proteins under flow rates up to 15 ml/ cm²·min and pressures up to 150 p.s.i. for production-scale applications.

Overview

• Easy to use—no optimization required
• Purifies more protein—high binding capacity (>10 mg of recombinant GST fusion protein per ml of resin)
• Yields highly purified protein—eluted protein is free of cellular contaminants
• Reusable—easy protocol for regenerating and storing resin
• Save money—30–40% less expensive than competing brands

Applications

• Purification of GST-tagged proteins: batch, gravity-flow, or FPLC applications

Reagents incompatible with Glutathione-Superflow Resin

These reagents are incompatible at any concentration:

• CHAPS
• DTE (dithioerythritol) and DTT (dithiothreitol)
• Guanidine hydrochloride
• SDS
• Urea