GST Purification Kit
GST Purification Kit
Brand: Takara Bio.
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GST Purification Kit
GST-tagged protein purification kit
The GST Purification Kit contains sufficient buffers and Glutathione-Superflow Columns for performing five batch/gravity-flow purifications. Up to 10 mg per prep of GST-tagged recombinant proteins can be purified using this kit.
Overview
- Easy to use—no optimization required
- Purifies more protein—high binding capacity (>10 mg of recombinant GST fusion protein per ml of resin)
- Yields highly purified protein—eluted protein is free of cellular contaminants
- Reusable—easy protocol for regenerating and storing resin
- Save money—30–40% less expensive than competing brands
Applications
- Purification of GST-tagged proteins: batch, gravity-flow, or FPLC applications
Reagents incompatible with Glutathione-Superflow Resin
These reagents are incompatible at any concentration:
- CHAPS
- DTE (dithioerythritol) and DTT (dithiothreitol)
- Guanidine hydrochloride
- SDS
- Urea
GST-tagged protein purification from whole cell extracts
GST-tagged protein purification from whole cell extracts. Whole cell extracts containing GST-DHFR (Panel A) and GST alone (Panel D) were loaded, washed, and eluted from glutathione resin columns. Then, the resulting purification fractions were analyzed by SDS-PAGE (Panels B & E) and Western blot with an anti-GST IgG (Panels C & F).
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GST-tagged protein purification from whole cell extracts
GST-tagged protein purification from whole cell extracts. Whole cell extracts containing GST-DHFR (Panel A) and GST alone (Panel D) were loaded, washed, and eluted from glutathione resin columns. Then, the resulting purification fractions were analyzed by SDS-PAGE (Panels B & E) and Western blot with an anti-GST IgG (Panels C & F).
