GAL4 AD and GAL4 DNA-BD Monoclonal Antibodies bind specifically to the major activation domain (AD) of GAL4 yeast two-hybrid prey proteins, and the DNA-binding domain (DNA-BD) of the bait protein, respectively.
If your bait vector is pGBKT7, your fusion protein can be detected with the GAL4 DNA-BD Monoclonal Antibody (our monoclonal c-Myc Antibody may also be used).
If your prey vector is pGADT7, pGADT7-Rec, or pACT2, the prey fusion protein can be detected with the GAL4 AD Monoclonal Antibody (our polyclonal HA-Tag Antibody may also be used).
Overview
Confirm expression of your bait protein
Sensitive monoclonal antibodies
Detect the GAL4 binding domain and the GAL4 activation domain
GAL4 AD and GAL4 DNA-BD Monoclonal Antibodies bind specifically to the major activation domain (AD) of GAL4 yeast two-hybrid prey proteins, and the DNA-binding domain (DNA-BD) of the bait protein, respectively.
If your bait vector is pGBKT7, your fusion protein can be detected with the GAL4 DNA-BD Monoclonal Antibody (our monoclonal c-Myc Antibody may also be used).
If your prey vector is pGADT7, pGADT7-Rec, or pACT2, the prey fusion protein can be detected with the GAL4 AD Monoclonal Antibody (our polyclonal HA-Tag Antibody may also be used).
Overview
Confirm expression of your bait protein
Sensitive monoclonal antibodies
Detect the GAL4 binding domain and the GAL4 activation domain