G418
For the selection and maintenance of eukaryotic cells which are stably transfected with vectors that express the neomycin resistance gene. Susceptibility of cells to G418 will vary with the cell type, media, growth conditions, degree of confluency for adherent cells, metabolic rate, and position in the cell cycle; therefore, we suggest performing a full dose response curve for each cell system used. Effective concentrations have been reported from 100 μg/ml up to greater than 5 mg/ml. Solutions of G418 should be filter-sterilized for use in tissue culture. This is the 1 g format. For 1 g format see Cat. # 631308.
Overview
- The most widely used selection agents for transfected mammalian cells carrying the resistance genes NeoR, HygR, and PuroR/Pac respectively
- G418 sulfate: An aminoglycoside antibiotic similar to gentamycin. Toxic to bacteria, yeast, higher plants, and mammalian cells, in addition to protozoans and helminths
- Hygromycin B: An aminoglycoside antibiotic that inhibits protein synthesis in bacteria, fungi, and higher eukaryotes
- Puromycin dihydrochloride: A broad spectrum antibiotic that inhibits protein synthesis in both prokaryotic and eukaryotic organisms
Properties
- G418 sulfate
- Molecular weight: 692.7
- Chemical formula: C20H40N4O10·2H2SO4
- Form: white to off-white powder
G418, Hygromycin B, and Puromycin
G418, Hygromycin B, and Puromycin. Recommended dose for selection and maintenance in mammalian cell lines.
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G418, Hygromycin B, and Puromycin
G418, Hygromycin B, and Puromycin. Recommended dose for selection and maintenance in mammalian cell lines.
