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FokI
FokI restriction site:
GGATG(N)9 |
CCTAC(N)13 |
Supplied buffer: M + BSA
Effect of DNA methylation: enzyme activity is not affected by CG methylase
Reaction temperature: 37°C
- Source: Escherichia coli UT 481 carrying the plasmid encoding the fokI gene
- Concentration: 4–12 units/µl
- Substrate for unit definition: lambda DNA
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
FokI restriction site:
GGATG(N)9 |
CCTAC(N)13 |
Supplied buffer: M + BSA
Effect of DNA methylation: enzyme activity is not affected by CG methylase
Reaction temperature: 37°C
- Source: Escherichia coli UT 481 carrying the plasmid encoding the fokI gene
- Concentration: 4–12 units/µl
- Substrate for unit definition: lambda DNA
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
