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FbaI (BclI)
FbaI (BclI) restriction site:
T | GATC A
A CTAG | T
Supplied buffer: K
Effect of DNA methylation: enzyme activity is affected by dam methylase; therefore, most DNAs from E. coli are not clevable.
Reaction temperature: 37°C
- Source: Flavobacterium balustinum
- Concentration: 4–12 units/µl
- Substrate for unit definition: N6-methyladenine free lambda DNA
- Star activity: unrelated site may often be cut in the presence of high concentration of glycerol or DMSO, and at alkaline pH or low ionic strength
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
FbaI (BclI) restriction site:
T | GATC A
A CTAG | T
Supplied buffer: K
Effect of DNA methylation: enzyme activity is affected by dam methylase; therefore, most DNAs from E. coli are not clevable.
Reaction temperature: 37°C
- Source: Flavobacterium balustinum
- Concentration: 4–12 units/µl
- Substrate for unit definition: N6-methyladenine free lambda DNA
- Star activity: unrelated site may often be cut in the presence of high concentration of glycerol or DMSO, and at alkaline pH or low ionic strength
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
