ExpressHyb Hybridization Solution
ExpressHyb Hybridization Solution
Brand: Takara Bio.
In stock
SKU
ExpressHyb Hybridization Solution
ExpressHyb Hybridization Solution reduces hybridization times while increasing sensitivity (Yang and Kain 1995). Other hybridization solutions typically require overnight incubations and often result in high background. Our hybridization solution can be used effectively with either radioactively or nonradioactively labeled DNA or oligonucleotide probes or riboprobes.
ExpressHyb Hybridization Solution is provided as a premade aqueous solution which is less viscous than other commercially available hybridization solutions and can be stored at room temperature. Complete Northern, Southern, and colony hybridization protocols for radioisotopic, colorimetric, and chemiluminescent detection systems are included with each shipment.
Overview
- Use with either radioactively or nonradioactively labeled DNA or oligonucleotide probes or riboprobes
- Reduces hybridization times while increasing sensitivity
Applications
- Ideal for use in all hybridization applications—northern blots, Southern blots, dot blots, cancer & disease profiling arrays, and colony hybridizations
- Optimized for use with both RNA & cDNA blots
ExpressHyb Hybridization Solution reduces background for a clean, sensitive hybridization
ExpressHyb Hybridization Solution reduces background for a clean, sensitive hybridization. The Human 12-Lane MTN Blot was hybridized with a radioactively labeled human transferrin receptor cDNA probe in ExpressHyb Solution at 60°C for 1 hr followed by a 15-hr exposure time.
Detection of gene expression levels in human placenta using ExpressHyb Hybridization Solution
Detection of gene expression levels in human placenta using ExpressHyb Hybridization Solution. The Atlas Human cDNA Expression Array was hybridized overnight and exposed to x-ray film for 2 days. The Atlas Human Array contains carefully selected cDNA fragments from 588 key genes for sensitive assessments of differential gene expression.
Yang, T. T. & Kain, S. R. Fast hybridization solution for the detection of immobilized nucleic acids. Biotechniques 18, 498-503 (1995).
Write Your Own Review
ExpressHyb Hybridization Solution reduces background for a clean, sensitive hybridization
ExpressHyb Hybridization Solution reduces background for a clean, sensitive hybridization. The Human 12-Lane MTN Blot was hybridized with a radioactively labeled human transferrin receptor cDNA probe in ExpressHyb Solution at 60°C for 1 hr followed by a 15-hr exposure time.
Detection of gene expression levels in human placenta using ExpressHyb Hybridization Solution
Detection of gene expression levels in human placenta using ExpressHyb Hybridization Solution. The Atlas Human cDNA Expression Array was hybridized overnight and exposed to x-ray film for 2 days. The Atlas Human Array contains carefully selected cDNA fragments from 588 key genes for sensitive assessments of differential gene expression.
