EpiXplore Methylated DNA Enrichment Kit
EpiXplore Methylated DNA Enrichment Kit
Brand: Takara Bio.
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EpiXplore Methylated DNA Enrichment Kit
The EpiXplore Methylated DNA Enrichment Kit provides a simple magnetic bead-based method for enriching and fractionating hypo- and hypermethylated DNA fragments from the whole genome. Enrichment leads to superior results in downstream applications that enhance the broader understanding of gene regulation, such as mapping methylation sites, by reducing background during sequencing. This is the 10 rxn version of this kit. For the 20 rxn version see Cat. # 631962.
Overview
- A simple magnetic bead-based method for enriching methylated DNA from the whole genome
- Retain >95% of methylated DNA
- Enriched DNA is immediately ready for downstream sequencing
- Protocol allows for gradient separation of low, medium, and highly methylated DNA
Applications
- Mapping methylation sites
- Methylation-sensitive restriction enzyme-based assays
- Real time and endpoint PCR assays
- Bisulfite sequencing
- Library preparation for high-throughput sequencing
- Sample prep for DNA microarray analysis
Methylated DNA enrichment protocol overview
Methylated DNA enrichment protocol overview.
Highly sensitive methylated DNA enrichment
Highly sensitive methylated DNA enrichment. The kit enriches DNA fragments containing ≥ 3 methylated CpG pairs (6 total methylation sites). FT: Flow-through containing unbound (non- or hypomethlyated) DNA. W: Wash. EL: Elution fraction containing methylated DNA.
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Methylated DNA enrichment protocol overview
Methylated DNA enrichment protocol overview.
Highly sensitive methylated DNA enrichment
Highly sensitive methylated DNA enrichment. The kit enriches DNA fragments containing ≥ 3 methylated CpG pairs (6 total methylation sites). FT: Flow-through containing unbound (non- or hypomethlyated) DNA. W: Wash. EL: Elution fraction containing methylated DNA.
